Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and acetoin from glucose.

Applied and Environmental Microbiology
H BoumerdassiGeorges Corrieu

Abstract

Following treatment with the mutagen N-methyl-N'-nitro-N-nitrosoguanidine, three mutants of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 that produced diacetyl and acetoin from glucose were isolated. The lactate dehydrogenase activity of these mutants was strongly attenuated, and the mutants produced less lactate than the parental strain. The kinetic properties of lactate dehydrogenase of strain CNRZ 483 and the mutants revealed differences in the affinity of the enzyme for pyruvate, NADH, and fructose-1,6-diphosphate. When cultured aerobically, strain CNRZ 483 transformed 2.3% of glucose to acetoin and produced no diacetyl or 2,3-butanediol. Under the same conditions, mutants 483L1, 483L2, and 483L3 transformed 42.0, 78.9, and 75.8%, respectively, of glucose to C4 compounds (diacetyl, acetoin, and 2,3-butanediol). Anaerobically, strain CNRZ 483 produced no C4 compounds, while mutants 483L1, 483L2, and 483L3 transformed 2.0, 37.0, and 25.8% of glucose to acetoin and 2,3-butanediol. In contrast to the parental strain, the NADH balance showed that the mutants regenerated most of the NAD via NADH oxidase under aerobic conditions and by ethanol production under anaerobic conditions.

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Citations

Jun 15, 2007·Critical Reviews in Microbiology·Zijun Xiao, Ping Xu
Jul 16, 2020·Applied Microbiology and Biotechnology·Juliana BleckwedelRaúl Ricardo Raya
Aug 31, 2000·Applied and Environmental Microbiology·J HugenholtzP Hols
Dec 16, 2003·The Journal of General and Applied Microbiology·Amnat PakdeetoSomboon Tanasupawat
Aug 19, 2014·Journal of the Science of Food and Agriculture·Yuxing GuoXiaoqun Zeng
May 26, 2001·Applied and Environmental Microbiology·N B JensenJ Villadsen
Jun 12, 2020·Frontiers in Microbiology·Jhonatan A Hernandez-ValdesOscar P Kuipers
Jun 1, 2002·Applied and Environmental Microbiology·Martin B PedersenDan Nilsson
Sep 14, 2021·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Jana ŠtefánikováMiroslava Kačániová

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