PMID: 8893Jul 1, 1976

Isolation and properties of yeast mutants with highly efficient thymidylate utilization

Zeitschrift Für Naturforschung. Section C: Biosciences
W W Fäth, M Brendel

Abstract

A screening procedure is presented which allows the isolation of yeast mutants (typ tir) with highly efficient utilization of exogenous deoxythymidine-5'-monophosphate (5'-dTMP) (greater than 50%). Data are given concerning the phenomenon of 5'-dTMP utilization in general: (i) The ability of S. cerevisiae to incorporate exogenous 5'-dTMP was found to already to be a wild type feature of this yeast, i.e. apparently not to be due to any mutation such as typ, tup, tmp, per or tum. Consequently these mutations are interpreted as amplifiers of a pre-given wild type potency. So far eight stages of 5'-dTMP utilization were detected as classified by the optimal 5'-dTMP requirement, with 5'-dTMP biosynthesis blocked, of the corresponding mutant strains isolated. All of them fit well into a mathematical series of the type "2n x 1.5" (n = 0, 1, 2, ..., 11), where the product term for n = 11 represents the 5'-dTMP requirement (mug/ml) of the best 5'-dTMP utilizing wild type strain found. (ii) Amplification of the 5'-dTMP utilizing potency obviously is due to any genetically determined alteration of the yeast 5'-dTMP uptaking principle itself or of physiological processes accompanying the monophosphate's uptake. (iii) The functioning of 5'-...Continue Reading

Citations

Oct 2, 1979·Molecular & General Genetics : MGG·R Fleer, M Brendel
Jan 1, 1982·Molecular & General Genetics : MGG·W W Fäth, M Brendel
Jan 1, 1982·Molecular & General Genetics : MGG·W W Fäth, M Brendel
Jan 1, 1981·Molecular & General Genetics : MGG·R ToperM Brendel

Related Concepts

Cistron
Science of Microbial Genetics
Hydrogen-Ion Concentration
Saccharomyces cerevisiae
Deoxythymidylate
Thymine Nucleotides

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