PMID: 3768416Nov 19, 1986Paper

Isolation and purification of a low-molecular-weight skeletal growth factor from human bones

Biochimica Et Biophysica Acta
S MohanD J Baylink

Abstract

A low-molecular-weight potent bone cell mitogen termed human skeletal growth factor (human SGF) was purified to homogeneity from human bone matrix. Extraction and initial purification steps were done under dissociative conditions to separate human SGF from high-molecular-weight complexes of bone matrix proteins. SGF activity was extracted from human femoral heads by demineralization with 10% EDTA in the presence of 4 M guanidine-HCl and proteinase inhibitors and was purified by hydroxyapatite, HPLC gel-filtration and HPLC reverse-phase chromatography. Human SGF thus purified was homogeneous by HPLC reverse-phase chromatography and SDS-polyacrylamide gel electrophoresis. The relative molecular mass of human SGF purified under dissociative conditions was 11,000. Human SGF stimulated bone cell proliferation ([3H]thymidine incorporation and cell number) at picomolar concentrations, with half maximum activity at 2-3 ng/ml (180-270 pM). Human SGF constitutes 0.00024% of organic bone matrix by weight.

References

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Nov 28, 1986·Biochimica Et Biophysica Acta·J E WergedalD J Baylink
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Citations

Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J E WergedalD J Baylink
Jul 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R D FinkelmanD J Baylink
Jan 1, 1988·The International Journal of Biochemistry·K H LauD J Baylink
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S MohanD J Baylink
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R D FinkelmanN H Bell
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R D FinkelmanD J Baylink
Dec 1, 1992·Pathology, Research and Practice·M H ZhengJ M Papadimitriou
Jan 1, 1992·Bone and Mineral·H C AndersonT Hunt
Jul 1, 1989·Journal of Cellular Physiology·S MohanD Baylink
May 15, 1997·Microscopy Research and Technique·J DequekerS Boonen
Aug 1, 1988·Journal of Oral Pathology·C EngströmD J Baylink
Dec 1, 1987·Acta Orthopaedica Scandinavica·J T Triffitt
Apr 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L PizurkiJ P Bonjour
Nov 28, 1986·Biochimica Et Biophysica Acta·J E WergedalD J Baylink
Jan 1, 1990·Metabolism: Clinical and Experimental·C M BautistaD J Baylink

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