PMID: 370Dec 1, 1975

Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans

Journal of Bacteriology
S EbisuA Misaki

Abstract

Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by S. mutans OMZ 176, which could be important in formation of dental plaques, was the most resistant. By enrichment culture of soil specimens, using OMZ 176 glucans as the sole carbon source, an organism was isolated that produced colonies surrounded by a clear lytic zone on opaque agar plates containing the OMZ 176 glucan. The organism was identified as a strain of Flavobacterium and named the Ek-14 bacterium. EK-14 bacterium was grown in Trypticase soy broth, and an enzyme capable of hydrolyzing the OMZ 176 glucan was concentrated from the culture supernatant and purified by negative adsorption on a diethylaminoethyl-cellulose (DE-32) column and gradient elution chromatography with a carboxymethyl-cellulose (CM-32) column. The enzyme was a basic protein with an isoelectric point of pH 8.5 and molecular weight of 65,000. Its optimum pH was 6.3 and its o...Continue Reading

Citations

Feb 21, 2007·Biotechnology Letters·M PleszczyńskaJ Szczodrak
Jun 20, 2012·Preparative Biochemistry & Biotechnology·M PleszczyńskaJ Szczodrak
Jan 1, 1992·Microbiology and Immunology·T IgarashiN Goto
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Wasana SuyothaMamoru Wakayama
May 2, 2006·Acta Biologica Hungarica·A WiaterM Pleszczyńska
Jul 28, 2016·Journal of Medical Microbiology·Sudheer Kumar BuddanaSambasiva Rao Raja Surya Krothapalli

Related Concepts

Cell-Free System
Dental Plaque
Flavobacterium genus
Exoglycosidases
Hydrogen-Ion Concentration
Hydrolysis
Metals
Polysaccharides
Polysaccharides, Bacterial
Soil Microbiology

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