PMID: 16524109Mar 10, 2006Paper

Isolation and purification of oridonin from Rabdosia rubescens using upright counter-current chromatography

Journal of Separation Science
Yanbin LuYuanjiang Pan

Abstract

A preparative counter-current chromatography (CCC) method for isolation and purification of oridonin, a new cancer chemoprevention agent, from the Chinese medicinal plant Rabdosia rubescens was successfully established. The crude oridonin was obtained by elution with a light petroleum/acetone solvent mixture from ethanol extracts of R. rubescens using column chromatography on silica gel. With a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1:2:1:2, v/v), 120 mg of oridonin at the purity of 97.8% was obtained from 200 mg of the crude sample in a single-step CCC separation. The structure of oridonin was identified by ESI-MS, 1H NMR, and 13C NMR.

References

Dec 14, 2004·The Journal of International Medical Research·J J LiuQ Lin

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Citations

May 24, 2008·Natural Product Reports·Otto Sticher
Nov 30, 2013·Bulletin of Experimental Biology and Medicine·G N Zyuz'kovA M Dygai
May 26, 2010·Journal of Natural Products·Ning KangTakashi Ikejima
Jan 16, 2015·Journal of Natural Products·Xu LiuHan-Dong Sun
Dec 6, 2019·Journal of the American Chemical Society·Lingran KongTuoping Luo

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