PMID: 6574305Jan 1, 1983Paper

Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation

Molecular & General Genetics : MGG
E J ZöllnerF P Schwindling


A rapid and easy final purification method is described for the isolation of plasmids from B. fragilis. Using RbTCA density gradient centrifugation in an airfuge ultracentrifuge ccc plasmid DNA can be separated from RNA, residual chromosomal DNA, linear and oc plasmid DNA. Pure ccc plasmid DNA is obtained from cultures of between 1 ml and 2 l in less than one day.


Nov 2, 1978·European Journal of Biochemistry·A ColmanA Lyons
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
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Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
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Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
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Nov 15, 1981·Analytical Biochemistry·P Stougaard, S Molin

Related Concepts

Bacteroides fragilis
Centrifugation, Density Gradient
DNA, Bacterial
Sodium Trichloroacetate

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