Isolation and purification of primary rodent astrocytes

Current Protocols in Neuroscience
D E Weinstein

Abstract

Astrocytes are a major cell type in the mammalian central nervous system (CNS). The ability to obtain virtually pure populations of these cells makes it possible to study their function as isolated cells or in mixed populations where they support the growth and survival of surrounding neurons. Unlike other mature CNS cells, mature astrocytes maintain the lifelong ability to reenter the cell cycle. The first isolation procedure described in this unit takes advantage of the proliferative ability of these cells, as does the second, except that no antibody or complement treatment is required. A procedure for detecting glial fibrillary acidic protein (GFAP), which is present in most astrocytes in vivo and virtually all astrocytes in vitro and is a useful marker for assessing the purity of cultures, is also presented.

References

Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
May 1, 1987·The Journal of Cell Biology·M E Hatten
Feb 1, 1985·The Journal of Cell Biology·M E Hatten

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Citations

Nov 20, 2014·Bulletin of Experimental Biology and Medicine·Ya A ZorkinaV P Chekhonin
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeppe FalsigMarcel Leist

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