Isolation and Purification of Primary Rodent Schwann Cells

Methods in Molecular Biology
Marta Palomo IrigoyenAshwin Woodhoo

Abstract

Schwann cells are the main glial cells of the peripheral nervous system (PNS) and play key roles in peripheral nerve development and function, including providing myelin that is essential for normal movement and sensation in the adult. Schwann cells can be readily destabilized by a wide variety of distinct conditions that range from nerve injury to immune assaults, metabolic disturbances, microbial infections, or genetic defects, leading to the breakdown of myelin (demyelination) and a subsequent switch in phenotypic states. This striking feature of Schwann cells forms the cornerstone of several debilitating and even fatal PNS neurological disorders that include the demyelinating neuropathies Guillain Barré syndrome (GBS) and Charcot-Marie-Tooth disease (CMT), and PNS cancers, including Neurofibromatosis.Primary Schwann cell cultures have proved a valuable tool to dissect key mechanisms that regulate proliferation, survival, differentiation, and myelination of these glial cell types. In this chapter, we describe the steps involved in the isolation and purification of Schwann cells from rodent peripheral nerves and the use of these cultures to model myelination in vitro.

Citations

Apr 17, 2019·Experimental and Therapeutic Medicine·Chu ChenTianmei Qian
May 16, 2019·Neural Regeneration Research·Xi-Meng JiTian-Mei Qian
Aug 22, 2021·Stem Cell Reviews and Reports·Liangfu JiangXiaofeng Jia

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