Isolation and Screening of Bacteria for Their Diazotrophic Potential and Their Influence on Growth Promotion of Maize Seedlings in Greenhouses

Frontiers in Plant Science
Medhin H Kifle, Mark D Laing

Abstract

Poor soil fertility is one of the major constraints for crop production. Nitrogen is the most limiting nutrient for increasing crop productivity. Therefore, there is a need to identify diazotrophic inoculants as an alternative or supplement to N-fertilizers for sustainable agriculture. In the current study, a number of free-living diazotrophic bacteria were isolated from soils collected from maize rhizosphere and from leaves and roots of maize within the KwaZulu-Natal Province, Republic of South Africa. Ninety-two isolates were selected for further screening because they were able to grow on N-free media containing different carbon sources. Isolates that were very slow to grow on N-free media were discarded. The isolates were screened in vitro for diazotrophic potential tests for ammonia production and acetylene reduction. Ethylene (C2H4) production was quantified and ranged from 4 to 73 nmoles of C2H4h(-1) culture(-1). The top 20 isolates were re-screened on maize seedlings, and eight isolates significantly (P = 0.001) enhanced some growth parameters of maize above the un-inoculated control. Isolates that showed significant effect on at least two growth parameters were identified at species or genera level. In conclusion, sele...Continue Reading

References

May 20, 1998·Trends in Microbiology·B Reinhold-Hurek, T Hurek
May 28, 2002·Canadian Journal of Microbiology·Paulina EstradaJesus Caballero-Mellado
Feb 14, 2004·Journal of Applied Microbiology·A KhalidZ A Zahir
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May 10, 2015·Applied and Environmental Microbiology·M A AbialaC B Blackwood

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Citations

Oct 8, 2016·Frontiers in Plant Science·Medhin H Kifle, Mark D Laing
Jan 26, 2018·Journal of Applied Microbiology·A Castellano-HinojosaN Santillana
Oct 19, 2021·Frontiers in Microbiology·Shabiha Nudrat HazarikaDebajit Thakur
Dec 2, 2021·ACS Synthetic Biology·Amy WenKarsten Temme

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