Isolation and screening of carboxydotrophs isolated from composts and their potential for butanol synthesis

Environmental Technology
Sean NguyenSonia M Tiquia-Arashiro

Abstract

Carboxydotrophs are known for their ability to convert carbon monoxide (CO) to butanol through fermentation. Such a platform offers a promising alternative approach to biofuel production from synthesis gas feedstocks. In this study, carboxydotrophs were isolated from various manure compost. Out of 500 isolates, only 11 carboxydotrophs (7 mesophiles and 4 thermophiles) were found to utilize CO as the sole source of carbon and energy. To assess the biochemical basis for their ability to produce biofuel (butanol), the level of activities of CO dehydrogenase (CODH), hydrogenase and butanol dehydrogenase (BDH) enzymes for these isolates against the known carboxydotroph, Butyribacterium methylotrophicum was assessed. All isolates showed evidence of enzyme activities (0.16-2.20 micromol min(-1)), with the majority exhibiting higher activities compared with the known carboxydotroph, B. methylotrophicum (0.33-0.71 micromol min(-1)). The level of activities for CODH and BDH ranged from 0.163-3.59 micromolmin(-1) and 0.19-2.2 micromolmin(-1), respectively. Three isolates (M7-1, T2-22, and T3-14) demonstrated enzymatic activity three to seven times higher than B. methylotrophicum. Of these, T2-22 exhibited the highest BDH activity and show...Continue Reading

References

Mar 1, 1983·Journal of Bacteriology·L H Lynd, J G Zeikus
Jan 1, 1983·Annual Review of Microbiology·O Meyer, H G Schlegel
Jun 1, 1980·Journal of Bacteriology·N L Schauer, J G Ferry
Apr 1, 1982·Journal of Bacteriology·N L Schauer, J G Ferry
Apr 1, 1993·International Journal of Systematic Bacteriology·R S TannerD Yang
Jan 5, 1999·Science·M W Adams, E I Stiefel
May 2, 2000·Applied and Environmental Microbiology·M J LoriteE J Bedmar
Dec 18, 2002·Journal of Bacteriology·Sae W ParkYoung M Kim
Mar 27, 2003·International Journal of Systematic and Evolutionary Microbiology·Philip Hugenholtz, Thomas Huber
Apr 20, 2004·Environmental Pollution·S M Tiquia, N F Tam
Jan 1, 1997·Environmental Pollution·S M TiquiaI J Hodgkiss
Jul 9, 2004·Applied and Environmental Microbiology·Kari E Dunfield, Gary M King
Sep 16, 2005·Journal of Applied Microbiology·S M Tiquia
Apr 6, 2006·Critical Reviews in Biotechnology·Jan SipmaAlfons J M Stams
Sep 14, 2006·Chemical Reviews·George W HuberAvelino Corma
Oct 21, 2006·Biotechnology and Bioengineering·Young S DoAlan A DiSpirito
Jan 17, 2007·Nature Reviews. Microbiology·Gary M King, Carolyn F Weber
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Apr 3, 2007·Current Opinion in Biotechnology·Anne M HenstraAlfons J M Stams
Aug 30, 2008·Biotechnology and Bioengineering·Sang Yup LeeKwang S Jung
Jul 4, 2009·FEMS Microbiology Ecology·Tatyana G SokolovaAlexander V Lebedinsky
Jan 26, 2010·Bioresource Technology·Pradeep Chaminda Munasinghe, Samir Kumar Khanal
Dec 3, 2010·PloS One·Jon M KuchenreutherJames R Swartz
Mar 1, 2011·Current Opinion in Biotechnology·Michael KöpkeSéan D Simpson
Mar 4, 2011·Current Opinion in Biotechnology·Edward M Green
May 14, 2011·Current Opinion in Biotechnology·Peter Dürre
Sep 20, 2012·Environmental Technology·Kelly ChoSonia M Tiquia

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Citations

Jan 27, 2016·Environmental Technology·Eric Pomaranski, Sonia M Tiquia-Arashiro
Sep 10, 2020·FEMS Microbiology Reviews·Florian Kremp, Volker Müller

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Methods Mentioned

BETA
light microscopy
PCR

Software Mentioned

Check
BLAST
Chimera

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