Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli (EHEC and STEC) in cattle

Journal of Applied Microbiology
Cecilia Dini, Patricio J de Urraza

Abstract

To isolate, characterize and select phages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing Escherichia coli (EHEC and STEC) in cattle. Sixteen STEC and EHEC coliphages were isolated from bovine minced meat and stool samples and characterized with respect to their host range against STEC, EHEC and other Gram-negative pathogens; their morphology by electron microscopy; the presence of the stx1, stx2 and cI genes by means of PCR; RAPD and rep-PCR profiles; plaque formation; and acid resistance. Six isolates belonged to the Myoviridae and 10 to the Podoviridae families. The phages negative for stx and cI that formed large, well-defined plaques were all isolated using EHEC O157:H7 as host. Among them, only CA911 was a myophage and, together with CA933P, had the broadest host range for STEC and EHEC; the latter phage also infected Shigella and Pseudomonas. Isolates CA911, MFA933P and MFA45D differed in particle morphology and amplification patterns by RAPD and rep-PCR and showed the highest acidity tolerance. Myophage CA911 and podophages CA933P, MFA933P and MFA45D were chosen as the best candidates for biocontrol of STEC and EHEC in cattle. This work employs steps for a rational selection and characteri...Continue Reading

References

Apr 1, 1975·Journal of Virology·W BesslerS Stirm
Jul 1, 1991·Applied and Environmental Microbiology·H R SmithB Rowe
Mar 1, 1990·Journal of Clinical Microbiology·D R PollardK R Rozee
Aug 1, 1982·Journal of Virology·B KwiatkowskiS Stirm
Jul 2, 1998·Applied and Environmental Microbiology·Maite Muniesa, Juan Jofre
Oct 27, 1998·Molecular Ecology·T PérezA Domínguez
Sep 3, 1999·Applied and Environmental Microbiology·I T KudvaC J Hovde
Feb 24, 2001·Nature·N T PernaF R Blattner
Sep 7, 2001·Annual Review of Microbiology·W C Summers
Oct 31, 2002·Applied and Environmental Microbiology·Rodolphe BarrangouTodd R Klaenhammer
Feb 21, 2003·International Journal of Food Microbiology·C DontorouS Levidiotou
Apr 30, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Franz AllerbergerLothar B Zimmerhackl
Jun 5, 2003·Antonie van Leeuwenhoek·Maite MuniesaJuan Jofre
Jun 9, 2004·Applied and Environmental Microbiology·G O'FlynnAidan Coffey
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Sandra Chibani-ChennoufiHarald Brüssow
Sep 3, 2004·The Journal of Pediatrics·Ann E SalernoBernard S Kaplan
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Harald BrüssowWolf-Dietrich Hardt
Sep 15, 2004·International Journal of Food Microbiology·Lelis MeichtriMarta Rivas
Oct 6, 2004·Animal Health Research Reviews·Rodney A Moxley
Jul 8, 2005·Microbiology·Harald Brüssow
Oct 26, 2005·Journal of Bioscience and Bioengineering·Yasunori TanjiHajime Unno
Nov 1, 2005·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Shigenobu MatsuzakiShosuke Imai
Nov 16, 2005·Annual Review of Genetics·Amos B OppenheimSankar Adhya
Jun 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cory M RobinsonAlison D O'Brien
Aug 31, 2006·The Journal of Infectious Diseases·Mark E WickhamB Brett Finlay
Jun 15, 2007·Journal of Theoretical Biology·Stephen T Abedon, Rachel R Culler
Oct 9, 2007·Journal of Theoretical Biology·Stephen T Abedon, Rachel R Culler
Apr 3, 2008·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Andrew M Kropinski
Apr 29, 2008·Emerging Infectious Diseases·Marta RivasPatricia M Griffin
Jun 26, 2008·European Journal of Pediatrics·Sally Johnson, C Mark Taylor
Aug 30, 2008·Applied and Environmental Microbiology·A E MatherD J Mellor
Nov 15, 2008·Nature Reviews. Microbiology·Margo Chase-ToppingMark Woolhouse
Dec 24, 2008·Animal Health Research Reviews·R P JohnsonA M Donoghue
Jan 24, 2009·Trends in Microbiology·Rodney M Donlan
Apr 3, 2009·Virology·Emmanuel DenouSophie Zuber
May 7, 2009·FEMS Microbiology Reviews·Sarah O'FlahertyAidan Coffey

Citations

Dec 14, 2011·Animal Health Research Reviews·J M SoonR N Baines
Mar 19, 2013·Virology Journal·Andrew M KropinskiR P Johnson
Aug 29, 2014·Applied Biochemistry and Biotechnology·Cecilia DiniGuillermo R Castro
Apr 9, 2011·International Journal of Food Microbiology·Michael P Doyle, Marilyn C Erickson
Feb 15, 2011·International Journal of Microbiology·Veronica CasasStanley R Maloy
Jun 23, 2011·FEMS Microbiology Letters·Diana GutiérrezBeatriz Martínez
Mar 13, 2016·Journal of Applied Microbiology·Cecilia DiniPatricio J de Urraza
Apr 20, 2018·Advanced Pharmaceutical Bulletin·Karla Veloso Gonçalves RibeiroLeandro Licursi de Oliveira
Jun 13, 2020·BMC Microbiology·Dragica DamnjanovicWallace J Bridge
Jan 25, 2019·Gels·Víctor H Pérez-Luna, Orfil González-Reynoso

Related Concepts

Bos indicus
Coliphages
Feces
Genes, Viral
Meat
Nested Polymerase Chain Reaction
Virulence Factors
Shiga-Toxigenic Escherichia coli
Enterohemorrhagic Escherichia coli
Host Specificity

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