May 1, 1975

Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation

The Journal of Cell Biology
L J RomrellS Ito


Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, which are larger than the other cell types, in fractions with purity of 75-95%. The homogeneity of cell fractions has been assessed by light and electron microscopy. The ability of the isolated cells to exclude the dye trypan blue, to incorporate labeled substrate, to consume oxygen, and to retain their structural integrity indicates that they are viable and still capable of functional activity.

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Mentioned in this Paper

Centrifugation, Density Gradient
Structure of Pyloric Gland
Electron Microscopy, Diagnostic
Electron Microscopy
Parietal Cells, Gastric
Sedimentation Procedure
Microscopy, Differential Interference Contrast
Cell Fraction
Cell Type

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