Abstract
Three new venom peptides were isolated from the Brazilian wasp; Protonectarina sylveirae and their complete amino acid sequences were determined by Edman degradation as well as by FAB-mass spectrometry. One (P-8) of them was a new analog of mastoparan family; therefore, we named it Protonectarina mastoparan. Another peptide (P-10) had an amino acid sequence homology with Ves-CP-T, a chemotactic peptide of wasp venom, and bombolitin-V, the venom peptide from bumblebee. The third peptide (P-6) was structurally unique, possessing an intramolecular disulfide bond. Not only Protonectarina mastoparan (P-8) but also P-6 and P-10 caused histamine release from rat peritoneal mast cells as potently as mastoparan (EC50s were about 1 x 10(-6) M for P-6 and 2 x 10(-6) M for P-10). In addition, P-10 in a concentration higher than 1 x 10(-5) M induced hemolysis though whose hemolytic activity was about a half potency of that of mastoparan. However, P-6 did not cause hemolysis up to the concentration of 10 microM. We have named them sylverin for the peptide P-6 and protonectin for the peptide P-10.
References
Aug 1, 1979·Chemical & Pharmaceutical Bulletin·Y HiraiT Nakajima
Jan 1, 1985·Peptides·T NakajimaY Tsukamoto
Feb 1, 1985·European Journal of Biochemistry·T TakaoY Shimonishi
Jul 28, 1972·Science·E Habermann
Jan 16, 1981·Journal of Chromatography·Y IshidaK Asai
Citations
Aug 13, 2008·Amino Acids·Kazuya MurataTerumi Nakajima
Feb 1, 2006·Journal of the American Society for Mass Spectrometry·Stefano TurillazziLeonardo Dapporto
Apr 25, 2000·Toxicon : Official Journal of the International Society on Toxinology·K KonnoY Nakata
Feb 24, 2010·Amino Acids·P BrigatteMario Sérgio Palma
Jan 29, 2010·Amino Acids·Bibiana Monson de SouzaMario Sergio Palma
Jun 23, 2011·Journal of Proteomics·Nicoli Barão Baptista-SaidembergMario Sergio Palma
Apr 4, 2015·Toxins·Miguel Moreno, Ernest Giralt
Jan 26, 2016·Toxins·Si Hyeock LeeKyungjae Andrew Yoon
Oct 18, 2012·Journal of Insect Physiology·Sébastien J M Moreau
Mar 18, 2015·Biochemistry·Gisele BaldisseraJosé Roberto Ruggiero
Mar 5, 2016·Toxins·Helen Andrade ArcuriMario Sergio Palma
Jul 1, 2004·Toxicon : Official Journal of the International Society on Toxinology·Maria Anita MendesMario Sergio Palma
Sep 11, 2014·Chembiochem : a European Journal of Chemical Biology·Yen-Chu LinGisbert Schneider
Dec 5, 2006·Biological & Pharmaceutical Bulletin·Kazuya MurataTerumi Nakajima
Sep 10, 2015·Rapid Communications in Mass Spectrometry : RCM·Axel TouchardPierre Escoubas
Sep 2, 2020·Toxins·Andréia Mayer BiolchiMárcia Renata Mortari
May 2, 2018·Toxicon : Official Journal of the International Society on Toxinology·José Roberto Aparecido Dos Santos-PintoMario Sergio Palma
Jun 6, 2021·Journal of Colloid and Interface Science·Danubia Batista MartinsMarcia Perez Dos Santos Cabrera
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Maria Anita MendesMario Sergio Palma
Sep 1, 2005·Peptides·Bibiana M SouzaMario S Palma
Jul 6, 2010·Toxicon : Official Journal of the International Society on Toxinology·Nicoli B Baptista-SaidembergMario S Palma
Oct 28, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ji Hyeong Baek, Si Hyeock Lee
Jan 26, 2010·Toxicon : Official Journal of the International Society on Toxinology·Ji Hyeong Baek, Si Hyeock Lee