Isolation and sequence of an interferon-tau-inducible, pregnancy- and bovine interferon-stimulated gene product 15 (ISG15)-specific, bovine ubiquitin-activating E1-like (UBE1L) enzyme.

Biology of Reproduction
Lea A RempelT R Hansen

Abstract

Bovine (bov) interferon-stimulated gene product 15 (ISG15) is produced in the endometrium in response to conceptus-secreted interferon (IFN)-tau. ISG15 conjugates to endometrial proteins through an enzymatic pathway that is similar to ubiquitinylation. Ubiquitin-activating enzyme 1-like protein (UBE1L) initiates enzymatic conjugation by forming a thioester bond with ISG15, thus preparing it for transfer to the next series of enzymes. The bovUBE1L has not been described. We hypothesized that bovUBE1L was induced by pregnancy and IFN-tau in the endometrium. A 110-kDa protein was purified from bovine endometrial (BEND) cells based on affinity with recombinant (r) glutathione S-transferase (GST)-ISG15. This protein was digested in gel with trypsin. Seven peptides were purified using HPLC, sequenced using liquid chromatography-mass spectroscopy-mass spectroscopy and found to share 43-100% identity with human UBE1L. The full-length bovUBE1L cDNA was isolated from a BEND cell cDNA library, sequenced, and found to share 83% identity with human UBE1L cDNA. Northern blot revealed two mRNAs that were detected in greater (P<0.05) concentrations in endometrium from Day 17-21 pregnant versus nonpregnant cows. Western blots using antihuman UB...Continue Reading

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Citations

Dec 17, 2009·Reproduction, Fertility, and Development·C E FarinP W Farin
Nov 19, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Megan L ShoemakerThomas R Hansen
Feb 7, 2013·Reproductive Biology and Endocrinology : RB&E·Keiichiro KizakiKazuyoshi Hashizume
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas E SpencerEckhard Wolf
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Stefan BauersachsEckhard Wolf
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Yanming SunHaifu Lu
Sep 21, 2013·Physiological Genomics·Jared J RomeroThomas R Hansen
Apr 21, 2012·Biology of Reproduction·Solomon MamoPat Lonergan
Oct 19, 2017·Biology of Reproduction·Jared J RomeroThomas R Hansen
Jun 15, 2021·Animal Reproduction·Cecilia Constantino RochaGuilherme Pugliesi
Oct 31, 2006·Biochemical and Biophysical Research Communications·Norie NakasatoSatoshi Inoue
Jan 17, 2007·Biochemistry·Christina M SorensenThomas R Hansen

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Methods Mentioned

BETA
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Software Mentioned

BLAST

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