Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene

Gene
M V CubellisM Rossi

Abstract

The gene lacS coding for a beta-galactosidase (beta Gal; EC 3.2.1.23) has been cloned from the thermoacidophilic archaebacterium Sulfolobus solfataricus, strain MT-4. It encodes a polypeptide chain of 489 amino acids (aa) (56,764 Da) in good agreement with the value directly measured for the enzyme (60 +/- 2 kDa per subunit). The aa composition of the enzyme and, in particular, its peculiarly low cysteine content (one Cys per subunit) has been confirmed; at the same time, it has been observed that the very low G + C content of the S. solfataricus genome strongly influences the codon usage preferences in the lacS sequence. There appears to be no evident similarity between this and the Escherichia coli lacZ sequence, thus suggesting that the two enzymes have analogous function, but are not homologous. By comparison with the published sequences of archaebacterial promoters, terminators and ribosome-binding sites, potential regulatory sites have been identified in the flanking regions of the S. solfataricus lacS gene.

References

Mar 25, 1986·Nucleic Acids Research·G WichA Böck
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D CueJ Konisky
Oct 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·J ButterworthW M McCrae
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A Young, R W Davis
Jan 1, 1983·Journal of Molecular Evolution·G G Lennon, N W Fraser
Feb 10, 1984·Nucleic Acids Research·R Nussinov

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Citations

Sep 6, 2000·Biotechnology and Bioengineering·R Montalvo-RodriguezP Blum
Jan 1, 1995·World Journal of Microbiology & Biotechnology·M CiaramellaM Rossi
Jan 1, 1995·World Journal of Microbiology & Biotechnology·C Leuschner, G Antranikian
Jun 11, 2008·Archives of Microbiology·Silvia Berkner, Georg Lipps
Dec 4, 2009·Extremophiles : Life Under Extreme Conditions·Albert F EllenArnold J M Driessen
Oct 20, 2005·Journal of Bioscience and Bioengineering·S EzakiT Imanaka
Apr 5, 2003·Applied and Environmental Microbiology·Benevides C C PesselaAlfonso V Carrascosa
Apr 5, 2005·Archaea : an International Microbiological Journal·Chiara SchiraldiMario De Rosa
Aug 12, 2014·Journal of Plant Physiology·Masahiro EdaKenneth C Gross
Dec 1, 1991·Current Opinion in Genetics & Development·W Zillig
Oct 14, 2004·Journal of Applied Microbiology·C S KimD-K Oh
Feb 11, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Masahiro EdaToshiji Tada
May 10, 2000·The Journal of Biological Chemistry·M MoracciM Rossi
Oct 6, 1997·FEMS Microbiology Letters·I S Sørensen, G Dandanell

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