Isolation and sequencing of the gene encoding Sp23, a structural protein of spermatophore of the mealworm beetle, Tenebrio molitor

Gene
X Feng, G M Happ

Abstract

The cDNA for Sp23, a structural protein of the spermatophore of Tenebrio molitor, had been previously cloned and characterized (Paesen, G.C., Schwartz, M.B., Peferoen, M., Weyda, F. and Happ, G.M. (1992a) Amino acid sequence of Sp23, a structure protein of the spermatophore of the mealworm beetle, Tenebrio molitor. J. Biol. Chem. 257, 18852-18857). Using the labeled cDNA for Sp23 as a probe to screen a library of genomic DNA from Tenebrio molitor, we isolated a genomic clone for Sp23. A 5373-base pair (bp) restriction fragment containing the Sp23 gene was sequenced. The coding region is separated by a 55-bp intron which is located close to the translation start site. Three putative ecdysone response elements (EcRE) are identified in the 5' flanking region of the Sp23 gene. Comparison of the flanking regions of the Sp23 gene with those of the D-protein gene expressed in the accessory glands of Tenebrio reveals similar sequences present in the flanking regions of the two genes. The genomic organization of the coding region of the Sp23 gene shares similarities with that of the D-protein gene, three Drosophila accessory gland genes and two Drosophila 20-OH ecdysone-responsive genes.

References

Jan 1, 1992·Annual Review of Entomology·G M Happ
Jul 20, 1990·Journal of Molecular Biology·F MaschatJ A Lepesant
Feb 10, 1989·Cell·M Beato
Dec 22, 1986·Nucleic Acids Research·P Bucher, E N Trifonov
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Jan 1, 1993·Insect Biochemistry and Molecular Biology·C AntoniewskiJ A Lepesant
Jan 1, 1996·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T YaginumaG M Happ

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Citations

Mar 27, 2002·Evolution; International Journal of Organic Evolution·Tina NilssonGoran Arnqvist
Apr 24, 2008·Biological Reviews of the Cambridge Philosophical Society·Tatyana Y Fedina, Sara M Lewis
Nov 18, 2006·Genome Génome / Conseil National De Recherches Canada·W Evan BraswellWillie J Swanson
Dec 1, 2005·Gene·Wensheng Qin, Virginia K Walker
Apr 11, 2018·Molecular Reproduction and Development·Andreas Borchel, Frank Nilsen
May 30, 2006·Molecular Biology and Evolution·José A AndrésRichard G Harrison

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