Isolation and sequencing of two alpha-globin genes alpha(A) and alpha(D) in pigeon and evidence for embryo-specific expression of the alpha(D)-globin gene

Biochemical and Biophysical Research Communications
T IkeharaH Takei

Abstract

By screening a pigeon genomic DNA library, we isolated a recombinant phage clone containing the alpha(A)-globin gene. The DNA sequence of the approximately 6kbp-long insert fragment of the phage clone was determined. The sequence suggested the existence of pigeon alpha(D)-globin gene located 3.1 kbp upstream from the alpha(A)-globin gene. The expression of the alpha(D)-globin in late embryo was also shown by the N-terminal amino-acid sequence of the intact globin chain. These results show that two adult alpha-globin genes, alpha(A) and alpha(D), exist in the pigeon genome, and the alpha(D)-globin is expressed at the late embryo stage. The stage-specific expression suggests the existence of regulatory elements and factors interacting to inhibit transcription at the adult stage.

Citations

Nov 16, 2005·Proteomics·David AgudoRafael Linares
Dec 17, 2014·Molecular Biology and Evolution·Juan C OpazoJay F Storz
Jan 18, 2018·Genome Biology and Evolution·Federico G HoffmannJuan C Opazo
Jan 29, 2011·The Journal of Experimental Biology·Jay F StorzHideaki Moriyama
Feb 28, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Barbara ManconiMariagiuseppina Pellegrini

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