Isolation and some properties of extracellular heat-stable lipases from Pseudomonas fluorescens strain AFT 36

The Journal of Dairy Research
P F Fox, L Stepaniak

Abstract

Aeration increased the growth and lipase production in milk by Pseudomonas fluorescens strain AFT 36, isolated from refrigerated bulk milk. A heat-stable lipase was isolated from a shaken milk culture of this microorganism by DEAE-chromatography and gel filtration in Sepharose 6B. The lipase-rich fraction from DEAE cellulose contained 3 lipases that were separated by gel filtration; only the principal lipase, which represented approximately 71% of total lipolytic activity, was characterized. The purified enzyme showed maximum activity on tributyrin at pH 8.0 and 35 degrees C; it had a Km on tributyrin of 3.65 mM and was inhibited by concentrations of substrate greater than approximately 17 mM. The enzyme was very stable over the pH range 6-9; it was relatively heat-labile in phosphate buffer in the temperature range 60-80 degrees C, where it was stabilized significantly by Ca2+. It was, however, very stable at 100-150 degrees C: the D values at 150 degrees C were approximately 22 s and 28 s in phosphate buffer and synthetic milk serum respectively; the corresponding Z values in the temperature range 100-150 degrees C were approximately 40 and approximately 42 degrees C and the Ea for inactivation were 7.65 X 10(4) J mol-1 and 6...Continue Reading

References

Feb 1, 1974·British Journal of Preventive & Social Medicine·R J PethybridgeJ G Fryer
Jan 1, 1969·Biochimica Et Biophysica Acta·M SémérivaP Desnuelle
Feb 1, 1969·Biochemical Pharmacology·M J Pitout
Jan 11, 1967·Biochimica Et Biophysica Acta·I M KhanK M Shahani
Sep 1, 1967·Journal of General Microbiology·J R Mencher, J A Alford
Sep 1, 1967·Journal of General Microbiology·R C LawrenceB Reiter
May 1, 1983·The Journal of Dairy Research·L Stepaniak, P F Fox
Jan 1, 1980·Applied and Environmental Microbiology·R E Andersson
Jan 1, 1972·Methods in Enzymology·R Fields

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Citations

Apr 13, 1999·Critical Reviews in Food Science and Nutrition·C A ThomsonG Mazza
Oct 9, 2012·Applied and Environmental Microbiology·Myunghan SonJung Hoon Ahn
Jul 25, 2013·Bioprocess and Biosystems Engineering·Dang-Thuan Tran, Jo-Shu Chang
Jan 5, 2008·Bioprocess and Biosystems Engineering·G Seghal KiranK Natarajaseenivasan
Nov 4, 2015·Food & Function·Laura SamsFrédéric Carrière
Jun 23, 2009·Biotechnology Advances·Fariha HasanAbdul Hameed
Jun 2, 2009·International Journal of Food Microbiology·Sophie MarchandJan De Block
Nov 1, 1986·The Journal of Applied Bacteriology·A W ShelleyI C MacRae
Jan 1, 1987·Journal of Basic Microbiology·B E Fischer, H P Kleber
Apr 24, 2007·Advances in Applied Microbiology·Peggy G Braun
Sep 6, 2000·Journal of Applied Microbiology·R Koka, B C Weimer
Mar 23, 2011·Journal of Molecular Microbiology and Biotechnology·Akanbi Taiwo OlusesanNazamid Saari
Jun 1, 1988·Journal of Dairy Science·T J RenG L Christen
May 1, 1987·The Journal of Dairy Research·A R BuckyD S Robinson
Dec 1, 1993·Applied and Environmental Microbiology·C DupuisP Boyaval
Aug 1, 1993·Enzyme and Microbial Technology·E J Gilbert

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