Isolation and structural characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl coenzyme A reductase

Plant Molecular Biology
C CaellesA Boronat

Abstract

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) catalyses the synthesis of mevalonate, the specific precursor of all isoprenoid compounds present in plants. We have characterized two overlapping cDNA clones that encompass the entire transcription unit of an HMG-CoA reductase gene from Arabidopsis thaliana. The transcription product has an upstream non-coding sequence of 70 nucleotides preceding an open reading frame of 1776 bases and a 3' untranslated region in which two alternative polyadenylation sites have been found. The analysis of the nucleotide sequence reveals that the cDNA encodes a polypeptide of 592 residues with a molecular mass of 63,605 Da. The hydropathy profile of the protein indicates the presence of two highly hydrophobic domains near the N-terminus. A sequence of 407 amino acids corresponding to the C-terminal part of the protein (residues 172-579), which presumably contains the catalytic site, shows a high level of similarity to the region containing the catalytic site of the hamster, human, yeast and Drosophila enzymes. The N-terminal domain contains two putative membrane-spanning regions, in contrast to the enzyme from other organisms which has seven trans-membrane region...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1975·Archives of Biochemistry and Biophysics·J D Brooker, D W Russell
Jan 1, 1986·Molecular Biology Reports·E W Khandjian
Sep 9, 1988·Science·K Verner, G Schatz
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M E BassonJ Rine
Nov 26, 1986·Biochemical and Biophysical Research Communications·R E Dugan, S S Katiyar
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Dec 28, 1984·Biochemical and Biophysical Research Communications·J Roitelman, I Shechter
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DeanJ Bedbrook

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Citations

Apr 1, 1995·Plant Molecular Biology·M K BhattacharyyaB A Stermer
Jun 1, 1994·Journal of Chemical Ecology·J Gershenzon
Aug 1, 1991·Lipids·T J BachA Wettstein
Aug 12, 2009·Applied Microbiology and Biotechnology·Remco MuntendamOliver Kayser
Mar 30, 2007·Lipids·Masashi Suzuki, Toshiya Muranaka
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M EnjutoA Boronat
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T NakashimaS Hata
Mar 11, 1990·Nucleic Acids Research
Jul 13, 2002·Plant Physiology·Timothy P DevarenneJoe Chappell
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Katsuyoshi KondoKazuko Oba
Mar 5, 2013·Annual Review of Plant Biology·Eva VranováWilhelm Gruissem
Dec 27, 2011·Progress in Lipid Research·Andréa HemmerlinThomas J Bach
Jun 28, 2011·Plant Signaling & Behavior·Meritxell Antolín-LloveraNarciso Campos
Mar 30, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying YuNaixing Ye
Mar 29, 1996·The Journal of Biological Chemistry·N CunilleraA Ferrer
Oct 2, 2019·Annals of Botany·Hui WeiQiang Zhuge

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