PMID: 2121479Oct 5, 1990Paper

Isolation and structure determination of the intact sialylated N-linked carbohydrate chains of recombinant human follitropin expressed in Chinese hamster ovary cells

European Journal of Biochemistry
K HårdJ F Vliegenthart

Abstract

Biologically active recombinant human follitropin has been expressed in Chinese hamster ovary cells. The carbohydrate chains of the recombinant glycoprotein hormone were enzymatically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. The oligosaccharides were separated from the N-deglycosylated protein by gel-permeation chromatography on Bio-Gel P-100, and fractionated by a combination of FPLC on Mono Q and HPLC on Lichrosorb-NH2. The structures of the carbohydrate chains were determined by 500- or 600-MHz 1H-NMR spectroscopy. The following types of carbohydrates occur: monosialylated diantennary (10%), disialylated diantennary (43%), disialylated tri-antennary (5%), trisialylated tri-antennary (13%), trisialylated tri'-antennary (8%), and tetrasialylated tetraantennary (12%) N-acetyllactosamine type of carbohydrate chains, all bearing exclusively alpha 2-3-linked N-acetylneuraminic acid (Neu5Ac). Previously, for pituitary follitropin mono-, di-, tri-, tri'-, and tetra-antennary oligosaccharides containing alpha 2-3- as well as alpha 2-6-linked Neu5Ac residues were reported. The bisecting GlcNAc residues present in native follitropin were not detected in the recombinant glycoprotein. Of the oligosaccharid...Continue Reading

References

Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J RyanH T Keutmann
May 2, 1986·European Journal of Biochemistry·J H MutsaersJ F Vliegenthart
Dec 1, 1986·Journal of in Vitro Fertilization and Embryo Transfer : IVF·G OelsnerA H DeCherney
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Citations

Apr 5, 2011·Analytical and Bioanalytical Chemistry·Josephine GrassFriedrich Altmann
Nov 6, 2009·Glycoconjugate Journal·Elizabeth Higgins
Jul 9, 2011·Reviews in Endocrine & Metabolic Disorders·George R Bousfield, James A Dias
Feb 1, 1992·Current Opinion in Biotechnology·D A Ashford
Sep 8, 2012·Journal of the American Chemical Society·Maciej A Walczak, Samuel J Danishefsky
Jun 17, 2003·Clinical Obstetrics and Gynecology·Howard D McClamrock
Sep 9, 2010·Biological & Pharmaceutical Bulletin·Shigehiro YanagiharaKazuhiro Kutsukake
Aug 31, 2004·Reproductive Biomedicine Online·Claus Yding AndersenMadelon van Wely
Feb 14, 2017·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Johannes F G Vliegenthart
Sep 10, 2015·The Cochrane Database of Systematic Reviews·Nienke S WeissMadelon van Wely
Dec 22, 2017·Critical Reviews in Biotechnology·Justin Bryan Goh, Say Kong Ng
Nov 21, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Nazareth LoretiStella Campo
Jan 17, 2019·The Cochrane Database of Systematic Reviews·Nienke S WeissMadelon van Wely
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
May 28, 2019·Endocrinology·George R Bousfield, David J Harvey
Apr 29, 2020·Journal of the Endocrine Society·Teresa ZariñánAlfredo Ulloa-Aguirre
Jul 10, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·M P Rose
Feb 14, 2008·Analytical Chemistry·Sergey Y VakhrushevJasna Peter-Katalinić

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