Isolation and transcriptomic analysis of Anopheles gambiae oenocytes enables the delineation of hydrocarbon biosynthesis

ELife
Linda GrigorakiH Ranson

Abstract

The surface of insects is coated in cuticular hydrocarbons (CHCs); variations in the composition of this layer affect a range of traits including adaptation to arid environments and defence against pathogens and toxins. In the African malaria vector, Anopheles gambiae quantitative and qualitative variance in CHC composition have been associated with speciation, ecological habitat and insecticide resistance. Understanding how these modifications arise will inform us of how mosquitoes are responding to climate change and vector control interventions. CHCs are synthesised in sub-epidermal cells called oenocytes that are very difficult to isolate from surrounding tissues. Here we utilise a transgenic line with fluorescent oenocytes to purify these cells for the first time. Comparative transcriptomics revealed the enrichment of biological processes related to long chain fatty acyl-CoA biosynthesis and elongation of mono-, poly-unsaturated and saturated fatty acids and enabled us to delineate, and partially validate, the hydrocarbon biosynthetic pathway in An. gambiae.

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Citations

May 29, 2021·Scientific Reports·Fabiola Claudio-PiedrasHumberto Lanz-Mendoza
Jul 28, 2021·Communications Biology·Kelsey L AdamsFlaminia Catteruccia
Aug 21, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·M Szymczak-CendlakM Słocińska

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Datasets Mentioned

BETA
AGAP007264
AGAP003600
AAEL003611
AGAP013219
AAEL013542
AAE004278
AAEL006824
AAEL004278
AGAP001899
PRJEB37240

Methods Mentioned

BETA
transgenic
Fluorescent Activated Cell Sorting
FACS
RNAseq
confocal microscopy
PCR

Software Mentioned

TREE
tximport
HMMER
IR
phytools
eggNOG mapper
GeneScript
R stats
MAFFT
gffread

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