PMID: 7371639Mar 1, 1980Paper

Isolation and translation in vitro of four related vitellogenin mRNAs of estrogen-stimulated Xenopus laevis

European Journal of Biochemistry
B K FelberR Weber

Abstract

Cloning of vitellogenin cDNA of Xenopus laevis revealed that vitellogenin is encoded in a small family of genes representing two distantly related main groups A and B, each comprising two more closely related subgroups A1, A2, and B1, B2 respectively. To characterize the proteins derived from these genes we have isolated the corresponding mRNAs by hybridizing, under stringent conditions, cytoplasmic poly(a)-containing RNA from the liver of estrogen-stimulated Xenopus to filter-bound cDNA clones containing sequences specific for all four vitellogenin genes. Hybridization of the isolated mRNAs with nick-translated cDNA clones revealed that contamination of the mRNAs by those of the other main group was less than 0.1%. Melting curves of the hybrids prepared with the isolated mRNAs and cDNA clones specific for the four vitellogenin genes showed that the isolated vitellogenin mRNAs are also specific for the four subgroups. Analysis of R loops formed between isolated mRNAs and cDNA clones representing the corresponding subgroup further indicated about 10% cross-contamination between the more closely related mRNAs. In a reticulocyte lysate each of the four mRNAs coded for a 200 000-Mr protein immunoprecipitable by monospecific vitello...Continue Reading

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Citations

Dec 1, 1981·Journal of Steroid Biochemistry·J R Tata
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W WahliF E May
Jan 1, 1982·DNA·T C JamesJ R Tata
Aug 25, 1982·Nucleic Acids Research·A A ProtterD L Williams
Aug 1, 1980·European Journal of Biochemistry·R B JaggiG U Ryffel
Jan 5, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Dae-Sik HwangJae-Seong Lee
Jun 10, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kyun-Woo LeeJae-Seong Lee
May 16, 1983·Biochemical and Biophysical Research Communications·S Y Wang, D L Williams
Apr 17, 1981·Science·W WahliR Weber

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