PMID: 9418973Jan 7, 1998Paper

Isolation and transplantation of multipotential populations of epidermal growth factor-responsive, neural progenitor cells from the canine brain

Journal of Neuroscience Research
E A MilwardI D Duncan

Abstract

Glial cell transplantation into myelin-deficient rodent models has resulted in myelination of axons and restoration of conduction velocity. The shaking (sh) pup canine myelin mutant is a useful model in which to test the ability to repair human myelin diseases, but as in humans, the canine donor supply for allografting is limited. A solution may be provided by self-renewing epidermal growth factor (EGF)-responsive multipotential neural progenitor cell populations ("neurospheres"). Nonadherent spherical clusters, similar in appearance to murine neurospheres, have been obtained from the brain of perinatal wildtype (wt) canine brain and expanded in vitro in the presence of EGF for at least 6 months. Most of the cells in these clusters express a nestin-related protein. Within 1-2 weeks after removal of EGF, cells from the clusters generate neurons, astrocytes, and both oligodendroglial progenitors and oligodendrocytes. Transplantation of lacZ-expressing wt neurospheres into the myelin-deficient (md) rat showed that a proportion of the cells differentiated into oligodendrocytes and produced myelin. In addition, cells from the neurosphere populations survived at least 6 weeks after grafting into a 14-day postnatal sh pup recipient an...Continue Reading

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Citations

Apr 6, 2011·Cytotechnology·Fei YinQing-San Zhu
Sep 10, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Tamir Ben-Hur
May 19, 2012·PloS One·Saafan Z MalikDeborah J Watson
Nov 28, 2012·American Journal of Veterinary Research·Ji-Hey LimChristopher L Mariani
Aug 25, 2010·The Veterinary Clinics of North America. Small Animal Practice·Natasha Olby
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Apr 27, 2007·Journal of the Neurological Sciences·W F Blakemore
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Nov 8, 2008·Annals of the New York Academy of Sciences·Tamir Ben-Hur, Steven A Goldman
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Mar 10, 2006·Pathologie-biologie·Michael F Clarke
Dec 17, 2014·PloS One·Cecilie Jonsgar SandbergIver A Langmoen
Aug 10, 2011·Veterinary Pathology·R M Walton
Oct 27, 1998·Journal of Neuroscience Research·S C ZhangI D Duncan
Jul 13, 2004·Journal of Neuroscience Research·Henry KlassenPhilip H Schwartz
Dec 3, 1999·Journal of Neuroscience Research·S VitryA Baron-Van Evercooren
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