Isolation and validation of a candidate Rsv3 gene from a soybean genotype that confers strain-specific resistance to soybean mosaic virus

Virology
Phu-Tri TranKook-Hyung Kim

Abstract

Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Merr.] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cDNA of the Glyma.14g204700 was successfully cloned from L29. Over-expression of Glyma.14g204700 in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g204700 is likely Rsv3 gene that confers strain-specific resistance to SMV.

Citations

Feb 13, 2020·Plants·Kristin WidyasariKook-Hyung Kim
Sep 29, 2019·Nature Communications·Kazuhiro IshibashiAkito Kaga
Jan 1, 2018·Uirusu·Kazuhiro Ishibashi, Masayuki Ishikawa
Apr 24, 2021·Frontiers in Plant Science·Brian T RossMichelle L Flenniken

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