Isolation and visualization of mouse placental hematopoietic stem cells

Current Protocols in Stem Cell Biology
Christos GekasHanna K A Mikkola

Abstract

This unit describes the isolation of hematopoietic stem cells (HSCs) from the mouse placenta. The placenta was recently identified as an important hematopoietic site that generates HSCs de novo and provides a transitory niche for a large pool of HSCs during midgestation. This protocol includes a dissection technique for murine placenta, the mechanical and enzymatic steps of placental tissue dissociation, and phenotypical identification and isolation of HSCs. It also contains a method for immunohistochemical analysis of placenta tissue sections to visualize developing HSCs in the placenta.

References

Sep 26, 2003·Development·Marcio Alvarez-SilvaFrançoise Dieterlen-Lièvre
Mar 2, 2005·Developmental Cell·Christos GekasHanna K A Mikkola
Mar 2, 2005·Developmental Cell·Katrin Ottersbach, Elaine Dzierzak
Sep 6, 2005·Experimental Hematology·Hanna K A MikkolaFrancoise Dieterlen-Lievre
Sep 14, 2006·Development·Hanna K A Mikkola, Stuart H Orkin

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