Isolation by solid-phase extraction and liquid chromatographic determination of mutagenic amines in beef extracts

Journal of Chromatography. a
M T GalceranL Puignou

Abstract

A solid-phase extraction method was successfully optimized for the isolation and preconcentration of five mutagenic amines, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole, 3-amino-1-methyl-5H-pyrido[4,3-b]indole, 2-amino-9H-pyrido[2,3-b]indole, 2-amino-3-methyl-9H-pyrido[2,3-b]indole and 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine, and two co-mutagens, 1-methyl-9H-pyrido[4,3-b]indole and 9H-pyrido[4,3-b]indole. Coupling of diatomaceous earth, propylsulphonyl silica gel, and octadecylsilane cartridges was used to separate selectively the imidazopyridine and indolpyridine derivatives from those of quinoxaline and quinoline. A method based on this sample preparation was applied to the determination of twelve heterocyclic amines and related substances in a commercial beef extract using HPLC with electrochemical and fluorescence detection. Good recovery values were obtained, ranging between 55 and 99%. The co-mutagens 1-methyl-9H-pyrido[4,3-b]indole (harman) and 9H-pyrido[4,3-b]indole (norharman) were found in the beef extract at levels of 110 and 53 ng g-1, respectively, and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-9H-pyrido[2,3-b]indole (A alpha C) were tentatively identified.

References

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Citations

Jun 5, 2007·Analytical and Bioanalytical Chemistry·M Murkovic
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·F J SantosA Ristic
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E BermudoM T Galceran
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·R BusquetsM T Galceran
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·W Pfau, K Skog
Sep 16, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K I SkogM I Jägerstad
Mar 9, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A SolyakovM Jägerstad
Nov 5, 2002·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Irena BaranowskaAnna Pielesz
Apr 23, 2013·Journal of Food Science·Monika Gibis, Jochen Weiss
Oct 18, 2008·Molecular Nutrition & Food Research·Rosa BusquetsMaria Teresa Galceran
Jan 22, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Olga ViegasIsabel M P L V O Ferreira
Mar 31, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·O ViegasI M P L V O Ferreira
Jul 2, 2003·Journal of Chromatography. a·F ToribioM T Galceran
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·P Pais, M G Knize
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·F ToribioL Puignou
Oct 28, 2008·Journal of Agricultural and Food Chemistry·Armindo MeloIsabel M P L V O Ferreira
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Jul 10, 2003·Journal of Agricultural and Food Chemistry·Elna PerssonKerstin Skog
May 29, 2008·Journal of Agricultural and Food Chemistry·Ute JautzGertrud Elisabeth Morlock

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