Abstract
We isolated oryctin, a 66-residue peptide, from the hemolymph of the coconut rhinoceros beetle Oryctes rhinoceros and cloned its cDNA. Oryctin is dissimilar to any other known peptides in amino acid sequence, and its function has been unknown. To reveal that function, we determined the solution structure of recombinant (13)C,(15)N-labeled oryctin by heteronuclear NMR spectroscopy. Oryctin exhibits a fold similar to that of Kazal-type serine protease inhibitors but has a unique additional C-terminal α-helix. We performed protease inhibition assays of oryctin against several bacterial and eukaryotic proteases. Oryctin does inhibit the following serine proteases: α-chymotrypsin, endopeptidase K, subtilisin Carlsberg, and leukocyte elastase, with K(i) values of 3.9 × 10(-10) m, 6.2 × 10(-10) m, 1.4 × 10(-9) m, and 1.2 × 10(-8) m, respectively. Although the target molecule of oryctin in the beetle hemolymph remains obscure, our results showed that oryctin is a novel single domain Kazal-type inhibitor and could play a key role in protecting against bacterial infections.
References
May 20, 1987·Journal of Molecular Biology·M FujinagaM N James
May 1, 1994·Journal of Biochemistry·M EguchiS Hara
Mar 1, 1994·Journal of Biomolecular NMR·D S Wishart, B D Sykes
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
May 2, 1997·Journal of Molecular Biology·B M Baker, K P Murphy
Sep 17, 1998·European Journal of Biochemistry·J YangM Yamakawa
Apr 23, 1999·Journal of Biomolecular NMR·G CornilescuA Bax
Jul 30, 1999·Developmental and Comparative Immunology·M R Kanost
Jul 30, 1999·Developmental and Comparative Immunology·S NatoriA Kobayashi
Nov 24, 1999·European Journal of Biochemistry·J IshibashiM Yamakawa
Apr 10, 2003·Journal of Molecular Biology·Thomas LauberUte C Marx
Jul 16, 2003·Biochemical and Biophysical Research Communications·Tetsuya TomieMinoru Yamakawa
Aug 21, 2003·The Journal of Biological Chemistry·Mitsuhiro TakedaIchio Shimada
Dec 3, 2003·Journal of Structural and Functional Genomics·JunGoo Jee, Peter Güntert
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Sep 27, 2008·Bioinformatics·L HolmA Schenkel
Citations
Jul 10, 2013·Journal of Bacteriology·Takuya MiyakawaMasaru Tanokura
Feb 28, 2016·Veterinary Parasitology·Zhaoxia ZhangYixin Lu
Aug 8, 2015·Toxins·Cen QianGong-Yin Ye
Oct 19, 2012·The FEBS Journal·Chrystelle DeracheChristine Kellenberger
Oct 1, 2013·Toxicon : Official Journal of the International Society on Toxinology·Bo Yeon KimByung Rae Jin
Nov 30, 2014·Fish & Shellfish Immunology·Venkatesh KumaresanJesu Arockiaraj
Nov 7, 2017·PloS One·Joaquin Alonso, Manuel Martinez
Nov 18, 2017·Scientific Reports·Lei YangGongyin Ye
Dec 11, 2019·BMC Genomics·Matan ShelomiLi-Yu Liu
Aug 27, 2021·Toxins·Chenglong GaoJuan Shi