PMID: 6230231Mar 1, 1984Paper

Isolation, characterisation and crystallization of deoxyribonuclease I from bovine and rat parotid gland and its interaction with rabbit skeletal muscle actin

European Journal of Biochemistry
V KreuderH G Mannherz

Abstract

A purification procedure is described yielding DNase I from bovine and rat parotid glands of high homogeneity. The apparent molecular masses of the DNases I isolated have been found by sodium dodecyl sulfate/polyacrylamide gel electrophoresis to be 34 and 32 kDa for bovine and rat parotid DNase I, respectively, and thus differ from the enzyme isolated from bovine pancreas (31 kDa). By a number of different criteria concerning their enzymic behaviour, the isolated enzymes could be clearly classified as DNases I, i.e. endonucleolytic activity preferentially on native double-stranded DNA yielding 5'-oligonucleotides, a pH optimum at about 8.0, the dependence of their enzymic activity on divalent metal ions, their inhibition by 2-nitro-5-thiocyanobenzoic acid and by skeletal muscle actin. Comparison of their primary structure by analysis of their amino acid composition and also two-dimensional fingerprints and isoelectric focusing indicate gross similarities between the enzymes isolated from bovine pancreas and parotid, but distinct species differences, i.e. between the enzymes isolated from bovine and rat parotid. All the DNases I are glycoproteins. From bovine parotid DNase I crystals suitable for X-ray structure analysis could b...Continue Reading

References

Apr 1, 1976·Cell·S E HitchcockU Lindberg
Feb 1, 1977·The Journal of Cell Biology·J P KraehenbuhlJ D Jamieson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1977·Journal of Biochemistry·A FunakoshiY Takagi
Jun 15, 1976·European Journal of Biochemistry·H Stadler, B Wittmann-Liebold
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, U Lindberg
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Jul 1, 1969·Analytical Biochemistry·H Eibl, W E Lands
Mar 1, 1980·European Journal of Biochemistry·H G MannherzE Nowak
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D SuckH G Mannherz
Aug 1, 1953·Biochimica Et Biophysica Acta·L CUNNINGHAM, M LASKOWSKI
Jan 1, 1962·Journal of Molecular Biology·F OOSAWA, M KASAI

Citations

Jul 27, 2001·Molecular Reproduction and Development·C M MottaS Filosa
May 1, 2007·Apoptosis : an International Journal on Programmed Cell Death·Dirk Eulitz, H G Mannherz
Sep 1, 1994·The International Journal of Biochemistry·M Malicka-BlaszkiewiczD Nowak
Dec 15, 2005·The International Journal of Biochemistry & Cell Biology·Markus NapireiH G Mannherz
Sep 29, 2004·Analytical Biochemistry·Vuokko Jansson, Kristian Jansson
Apr 5, 2011·International Journal of Dentistry·Ioanna D Daskala, Christina C Tesseromatis
Apr 20, 2004·Biochemical and Biophysical Research Communications·Muhammet BektaşEngin Bermek
Aug 14, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Junko FujiharaHaruo Takeshita
Nov 15, 1994·European Journal of Biochemistry·D ShiokawaS Tanuma
Oct 1, 2019·PloS One·Tonya M SantausChris D Geddes
Mar 29, 2003·Physiological Reviews·C G dos RemediosN J Nosworthy
Jul 31, 2019·International Journal of Molecular Sciences·Zuzana KubiritovaRoman Gardlik
Nov 5, 1997·Archives of Biochemistry and Biophysics·D ShiokawaS Tanuma

Related Concepts

Isoactin
Bos indicus
Polymorphism, Crystallization
Streptodornase
Endodeoxyribonuclease
Muscle
Parotid Gland
Plasma Protein Binding Capacity
Protein Conformation

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