Isolation, characterisation, and genome sequencing of Rhodococcus equi: a novel strain producing chitin deacetylase.

Scientific Reports
Qinyuan MaMin Wang

Abstract

Chitin deacetylase (CDA) can hydrolyse the acetamido group of chitin polymers to produce chitosans, which are used in various fields including the biomedical and pharmaceutical industries, food production, agriculture, and water treatment. CDA represents a more environmentally-friendly and easier to control alternative to the chemical methods currently utilised to produce chitosans from chitin; however, the majority of identified CDAs display activity toward low-molecular-weight oligomers and are essentially inactive toward polymeric chitin or chitosans. Therefore, it is important to identify novel CDAs with activity toward polymeric chitin and chitosans. In this study, we isolated the bacterium Rhodococcus equi F6 from a soil sample and showed that it expresses a novel CDA (ReCDA), whose activity toward 4-nitroacetanilide reached 19.20 U/mL/h during fermentation and was able to deacetylate polymeric chitin, colloidal chitin, glycol-chitin, and chitosan. Whole genome sequencing revealed that ReCDA is unique to the R. equi F6 genome, while phylogenetic analysis indicated that ReCDA is evolutionarily distant from other CDAs. In conclusion, ReCDA isolated from the R. equi F6 strain expands the known repertoire of CDAs and could be...Continue Reading

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Citations

Oct 20, 2020·Current Opinion in Chemical Biology·Sergi Pascual, Antoni Planas

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Datasets Mentioned

BETA
PRJNA526377

Methods Mentioned

BETA
chemical treatment
PCR

Software Mentioned

X2
Clustal
MEGA
BLAST
CAZy
ReCDA
Blastp
HiSeq Control Software ( HCS ) OLB

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