Isolation, characterization, and application of a novel specific Salmonella bacteriophage in different food matrices

Food Research International
Chenxi HuangXiaohong Wang

Abstract

Application of bacteriophages to eliminate foodborne pathogens in food matrices is an emerging research field. In this study, a promising phage candidate specific for Salmonella strains was screened and its ability to decrease Salmonella counts in some food, such as milk, sausage, and lettuce, was investigated. A total of 58 Salmonella phages were isolated from a wastewater treatment plant, sewage near a river, farm ditch near a lake, and poultry house. Among them, phages LPST10, LPST18, and LPST23 were highly efficient in infecting Salmonella Typhimurium ATCC 14028. In particular, phage LPST10 could infect all the tested Salmonella Typhimurium and Salmonella Enteritidis strains with high efficiency. Bacterial challenge tests revealed that phage LPST10 and its combination with phages LPST18 and LPST23 could consistently inhibit the growth of multiple strains. Phage LPST10 presented a lysis time of about 50 min with a burst size of 101 PFU/CFU, exhibited two distinct phases in the one-step growth curve, and was stable at a pH range of 3-13 that corresponds to the pH of most of the foods (pH 3.5-7.5) and at temperatures between 30 °C and 60 °C. Transmission electron microscopy demonstrated that phage LPST10 belongs to the Siphovi...Continue Reading

Citations

Feb 7, 2019·Frontiers in Microbiology·Diana GutiérrezPilar García
Feb 23, 2020·Current Microbiology·Min LuXinchun Liu
Nov 14, 2020·Current Opinion in Biotechnology·Michela Gambino, Lone Brøndsted
Jul 9, 2021·Frontiers in Microbiology·Yuting ShangJumei Zhang
Aug 18, 2021·Food Research International·Yating GuoJinquan Li
Aug 25, 2021·Critical Reviews in Microbiology·Paulina ŚliwkaAneta Skaradzińska
Nov 14, 2021·Food and Environmental Virology·Sefika EvranIsmail Hakki Boyaci

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