May 28, 2020

Isolation, Characterization and Chemical Synthesis of Large Spectrum Antimicrobial Cyclic Dipeptide (l-leu-l-pro) from Streptomyces misionensis V16R3Y1 Bacteria Extracts. A Novel 1 H NMR Metabolomic Approach

Antibiotics
Ilhem SaadouliHadda-Imene Ouzari

Abstract

Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensisV16R3Y1 isolated from the date palm rhizosphere (southern Tunisia). This strain presented a broad range of antifungal activity against Fusariumoxysporum, Aspergillusflavus, Penicilliumexpansum, Aspergillusniger, Candida albicans, Candidametapsilosis, and Candidaparapsilosis and antibacterial activity against human pathogenic bacteria, including Escherichiafergusonii, Staphylococcus aureus, Salmonella enterica, Enterococcus faecalis, Bacillus cereus and Pseudomonas aeruginosa. The purification procedure entailed ethyl acetate extract, silica gel column, and thin layer chromatography. Based on 1H NMR metabolomic procedure application, also supported by the GC-MS analysis, cyclic dipeptide (l-Leucyl-l-Proline) was identified as the major compound in the bioactive fraction. In order to confirm the identity of the active compound and to have a large quantity ...Continue Reading

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Mentioned in this Paper

Streptomyces species
Streptomyces misionensis
NMR-2 protein, C elegans
1H NMR
Isolation Aspects
Antifungal Agents
Protein Purification
Thin Layer Chromatography
Objective (Goal)
Study

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