PMID: 7023647Mar 15, 1981Paper

Isolation, characterization and clinical evaluation of a pancreas cancer-associated antigen

Cancer
T ShimanoT M Chu

Abstract

A pancreas cancer-associated antigen (PCAA) was identified and isolated from ascites fluid of human pancreatic cancer. Purified PCAA was homogeneous as determined by polyacrylamide gel electrophoresis. PCAA was a glycoprotein with a molecular weight of approximately 1,000,000 and consisted of 20% carbohydrates and 80% peptides, had an isoelectric point of 4.7, and migrated to alpha 2-beta region. It possessed a sedimentation coefficient of 14S and appeared to be a fibrous or fibroglobular protein. Immunoreactivity of PCAA was sensitive to proteolytic enzymes, perchloric acid, KSCN, glycine-HCl at pH 2.5, urea and lithium diiodosalicylate; and insensitive to neuraminidase or beta-glucosidase. Immunohistochemical technique revealed that PCAA was located in the cytoplasm of ductal epithelial cells of malignant pancreas. Using heteroantiserum raised against purified PCAA, horseradish peroxidase and CNBr-activated Sepharose 4B, an enzyme-immunoassay (EIA) for circulating PCAA has been developed. From a group of 40 healthy blood donors, an upper limit of 16.2 micrograms of PCAA/ml of serum has been tentatively determined. An elevated PCAA was shown in 67% (29/43) of patients with pancreas cancer, as well as in 30% (11/36) of lung can...Continue Reading

References

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Citations

Dec 24, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·R LoorT M Chu
Dec 29, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·M KitadaG Kosaki
Sep 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·J FurukawaT Mori
Mar 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ImokawaY Kajita
Jan 1, 1986·Cancer Investigation·M H TanT M Chu
Jun 15, 1990·International Journal of Cancer. Journal International Du Cancer·C K Ching, J M Rhodes
Feb 1, 1988·Japanese Journal of Cancer Research : Gann·R YoshimuraM Akagi
Jan 1, 1983·Annals of the New York Academy of Sciences·T ShimanoG Kosaki
Jan 1, 1983·Annals of the New York Academy of Sciences·S KawaH Fukui
Jul 1, 1986·The British Journal of Surgery·N H GilinskyI N Marks
Jan 1, 1983·Annals of the New York Academy of Sciences·C R Taylor
Aug 15, 1984·International Journal of Cancer. Journal International Du Cancer·E HollandeF Clemente
Jan 1, 1997·Journal of Clinical Laboratory Analysis·T M Chu
Jan 1, 1982·Digestive Diseases and Sciences·H Z KupchikV L Go
Sep 1, 1983·Digestive Diseases and Sciences·T T WhiteH Miyashita
Dec 1, 1984·World Journal of Surgery·I Ihse, G Isaksson
Dec 1, 1984·World Journal of Surgery·D K Podolsky

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