PMID: 8969466Jun 1, 1996Paper

Isolation, characterization and complete nucleotide sequence of a Galleria mellonella cDNA coding for the follicle cell-specific yolk protein, YP4

Insect Biochemistry and Molecular Biology
V S Rajaratnam

Abstract

An ovariole-specific yolk polypeptide cDNA from Galleria mellonella was isolated from a cDNA library constructed using vitellogenic ovariole poly (A)+ RNA, by differential screening with the ovariole and day-0 male pupal poly (A)+ RNA. The Ov11 cDNA hybridized to a 1.1 kb message from the ovariole but not from male pharate adults, ovariectomized pharate adults or day-5 last instar larvae. Developmental Northern analysis showed that this message is expressed only during vitellogenic stages. In situ hybridization of digoxigenin-dUTP labeled Ov11 cDNA to the day-8 pharate adult ovariole showed that only follicle cells express the Ov11 gene. The 960 bp Ov11 cDNA is nearly full-length, containing a single open reading frame coding for a 286 amino acid polypeptide with a hydrophobic signal sequence and two potential N-glycosylation and tyrosine sulfation sites. The fact that Ov11 cDNA codes for the 37 kDa ovariole-specific yolk protein, YP4, was confirmed by the N-terminal amino acid sequence analysis of purified YP4. Genomic Southern analysis suggests that the yp4 gene is a single copy gene and PCR analysis using the 5' and 3' end primers of the cDNA indicates that the yp4 gene is intronless within the coding region.

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Citations

Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Dec 17, 2008·Journal of Insect Physiology·Muhammad Tufail, Makio Takeda
Sep 16, 2008·Journal of Insect Physiology·Muhammad Tufail, Makio Takeda
Mar 27, 2015·Archives of Insect Biochemistry and Physiology·Cen QianChao-Liang Liu
Apr 20, 1999·Archives of Insect Biochemistry and Physiology·O P Perera, P D Shirk
Feb 26, 2021·Insect Science·Xia XuYong-Ping Huang

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