Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets

Journal of Endodontics
Negar HomayounfarAshraf F Fouad

Abstract

The ferret canine tooth has been introduced as a suitable model for studying dental pulp regeneration. The aim of this study was to isolate and characterize ferret dental pulp stem cells (fDPSCs) and their differentiation potential. Dental pulp stem cells were isolated from freshly extracted ferret canine teeth. The cells were examined for the expression of stem cell markers STRO-1, CD90, CD105, and CD146. The osteo/odontogenic and adipogenic differentiation potential of fDPSCs was evaluated. Osteogenic and odontogenic marker genes were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) on days 1, 4, and 8 after osteo/odontogenic induction of fDPSCs including dentin sialophosphoprotein (DSPP), dentin matrix protein-1, osteopontin, and alkaline phosphatase. Human dental pulp cells were used as the control. The results were analyzed using 3-way analysis of variance. fDPSCs were positive for STRO1, CD90, and CD105 and negative for CD146 markers with immunohistochemistry. fDPSCs showed strong osteogenic and weak adipogenic potential. The overall expression of DSPP was not significantly different between fDPSCs and human dental pulp cells. The expression of DSPP in osteo/odontogenic media was significantly hi...Continue Reading

References

Mar 8, 2000·European Journal of Oral Sciences·K GuR B Rutherford
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Jun 2, 2009·Journal of Endodontics·Jaruma Beau SakdeePeter V Hauschka
Oct 19, 2010·Journal of Endodontics·Flavio F DemarcoJacques E Nör
Jan 18, 2011·Journal of Endodontics·Mahmoud TorabinejadShahrokh Shabahang
Jul 19, 2011·Journal of Endodontics·Waruna Lakmal DissanayakaLijian Jin
Nov 14, 2012·Journal of Endodontics·Xiaofei ZhuWenhao Zhu
Mar 6, 2013·Journal of Endodontics·Kenneth M HargreavesFabricio B Teixeira
Sep 18, 2013·Journal of Endodontics·Ali NosratAshraf F Fouad
Mar 29, 2014·Journal of Endodontics·Mahmoud TorabinejadShahrokh Shabahang
Apr 5, 2014·Journal of Endodontics·Ashraf F Fouad, Prashant Verma
Jun 1, 2014·Journal of Dental Research·G T-J Huang, F Garcia-Godoy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.