Isolation, characterization, and evaluation of wild isolates of Lactobacillus reuteri from pig feces.

The Journal of Microbiology
Deog Yong LeeHan Sang Yoo

Abstract

Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2 approximately 10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.

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Citations

Jan 31, 2015·Pathogens·Fengjuan YangShiyan Qiao
Jan 1, 2016·Gut Pathogens·Senait AssefaGerwald A Köhler
May 9, 2015·Journal of Animal Science and Biotechnology·Chengli HouShiyan Qiao
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May 9, 2012·Regulatory Toxicology and Pharmacology : RTP·Mitchell L JonesSatya Prakash
Feb 22, 2012·Journal of the Science of Food and Agriculture·Riyazuddeen Khan Mahmooduzzafar
Jul 15, 2021·Animal Nutrition·Jie PengYanhua Huang
Aug 11, 2021·Journal of Microbiology and Biotechnology·Yoo Jin KwonChe Ok Jeon

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