Isolation, characterization, and expression of Le-msx, a maternally expressed member of the msx gene family from the glossiphoniid leech, Helobdella

Developmental Dynamics : an Official Publication of the American Association of Anatomists
V A MasterM Q Martindale

Abstract

The msx gene family is one of the most highly conserved of the nonclustered homeobox-containing genes. We have isolated an msx homolog (Le-msx) from the glossiphoniid leech, Helobdella robusta, and characterized its pattern of expression by whole mount in situ hybridization. In situ expression and reverse transcription polymerase chain reaction (RT-PCR) data results show that Le-msx is a maternal transcript initially uniformly distributed in the cortex of immature oocytes that becomes asymmetrically localized to the polar regions of the uncleaved zygote. This is the earliest reported expression for the msx gene family and the first maternally expressed homeodomain-containing transcription factor reported in annelids. During embryonic development, Le-msx is expressed in all 10 embryonic stem cells and their segmental founder cell descendants. At midembryonic stages, Le-msx is expressed in the expanding germinal plate. Le-msx is confined to the central nervous system and nephridia at late (stage 9) stages and subsequently disappears from nephridia. In addition, we present a phylogenetic hypothesis for the evolution of the msx gene family, including the identification of a putative C. elegans msx homolog and the realignment of the...Continue Reading

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Citations

Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·K J Peterson, E H Davidson
Jul 7, 2001·Evolution & Development·M J Kourakis, M Q Martindale
Jun 1, 1997·Molecular Phylogenetics and Evolution·S Q IrvineM Q Martindale
Nov 27, 1999·Mechanisms of Ageing and Development·E Richelle-Maurer, G Van de Vyver

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