Isolation, characterization, and fermentative pattern of a novel thermotolerant Prototheca zopfii var. hydrocarbonea strain producing ethanol and CO2 from glucose at 40 degrees C

Archives of Microbiology
Ryohei UenoShigeru Kimura

Abstract

A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus Prototheca. Moreover, it also evolved gas from sucrose after a time lag at 40 degrees C. Its taxonomic characteristics coincided with those of Prototheca zopfii var. hydrocarbonea, and phylogenetic analysis, based on a small-subunit (SSU) rDNA sequence, also revealed a close relationship between the two strains. D-lactic acid, ethanol, CO2 and a trace of acetic acid were produced from glucose, but L-lactic acid, formic acid, and H2 were not. At 25 degrees C, D-lactic acid and ethanol were produced in approximately equimolar amounts under N2/H2/CO2, whereas ethanol production was predominant under N2. More ethanol was produced at 40 degrees C than at 25 degrees C irrespective of the gas composition in the atmosphere. This is the first report on gas production from glucose and on the changes in the fermentative patterns as a function of temperature for the genus Prototheca.

Citations

Jan 19, 2012·Veterinary Clinical Pathology·Laura V LaneBrenda C Love
Apr 13, 2007·Clinical Microbiology Reviews·Cornelia Lass-Flörl, Astrid Mayr
Apr 25, 2008·Journal of Clinical Microbiology·Sara MarquesGertrude Thompson
Sep 8, 2011·Bioscience, Biotechnology, and Biochemistry·Ryohei UenoNaoto Urano
Jan 26, 2010·Research in Veterinary Science·Sara MarquesGertrude Thompson
Nov 19, 2018·Journal of Dairy Science·Tomasz JagielskiHenryk Krukowski
Jan 8, 2019·Journal of Dairy Science·Ho-Sung ParkSuk-Kyung Lim
Mar 21, 2009·Medical Mycology·Gertrude ThompsonJúlio Carvalheira
May 17, 2018·Medical Mycology·John R ToddR Scott Pore

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