PMID: 8965647Mar 1, 1996Paper

Isolation, characterization and in vivo analysis of the murine calbindin-D28K upstream regulatory region

Brain Research. Molecular Brain Research
O PavlouH T Orr

Abstract

The genomic locus containing the murine calbindin-D28K gene has been isolated and partially characterized. Genomic cloning revealed an exon/intron chromosomal structure very similar to the avian gene previously described. The ability of the calbindin-D28K upstream region to direct cell-specific expression was tested in vivo. Varying lengths of upstream sequence were used to drive expression of lacZ in transgenic mice. Characterization of 23 transgenic mouse lines revealed that even as much as 3.0 kb of upstream sequence was unable to direct expression independently of integration site effects, suggesting the absence of important elements. Despite the small number of expressing transgenic lines and the great variability, there was a tendency of cell specificity of transgene expression exhibited in distinct brain regions. In the cerebellum, Purkinje cell-specific expression was observed with the shortest (1.0 kb) upstream sequence tested. Specificity of transgene expression in Purkinje cells was abolished with longer portions of upstream sequence. The same observation was made for transgene expression in granule cells of the dentate gyrus, while the opposite effect was observed for expression in CA1 hippocampal cells. The absence...Continue Reading

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Citations

Dec 5, 1997·Brain Research. Molecular Brain Research·K I StraussD M Jacobowitz
Jul 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Uwe MaskosPhilippe Brûlet
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·D B Arnold, N Heintz
Aug 12, 2015·Biochemical and Biophysical Research Communications·Asghar HajibeigiOrhan K Öz
Apr 7, 2010·Expert Opinion on Biological Therapy·Youngbuhm HuhKwang-Soo Kim

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