PMID: 3771643Oct 1, 1986Paper

Isolation, characterization, and localization of the spanning protein from skeletal muscle triads

The Journal of Cell Biology
R M KawamotoA H Caswell

Abstract

A monoclonal antibody has been developed against the putative junctional protein or spanning protein (SP) from skeletal muscle triads. By immuno-affinity chromatography, we have purified this protein. The native protein has a molecular mass of 630-800 kD, as determined by gel filtration and rate zonal centrifugation. Within the limits of the methods used, the basic unit of the SP appears to be a dimer. In electron micrographs, it is shown to exhibit a circular profile with a diameter of approximately 100 A. In thin section analysis, the protein is frequently observed as parallel tracks of electron-dense particles bordering a translucent core. We suggest that the basic unit of the junctional structure is a dimer of 300-kD subunits and that four such entities constitute the intact SP. The purified protein has been used to develop polyclonal antibodies. By immunoelectron microscopy using immunogold probes, the SP has been localized to the junctional gap of the triad. By attaching the SP to an affinity resin, three proteins have been identified as forming associations with the SP. The Mrs of the proteins are 150, 62, and 38 kD; the 62-kD protein is calsequestrin.

References

Oct 1, 1976·Archives of Biochemistry and Biophysics·A H CaswellJ P Brunschwig
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Citations

Apr 1, 1989·Journal of Bioenergetics and Biomembranes·A H Caswell, N R Brandt
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·P Volpe
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·F A Lai, G Meissner
Jan 1, 1992·Progress in Biophysics and Molecular Biology·A F Dulhunty
Jul 1, 1987·The Journal of Cell Biology·C Franzini-ArmstrongE Varriano-Marston
May 1, 1995·The Journal of Cell Biology·X H SunC Franzini-Armstrong
Mar 21, 1998·The Journal of Cell Biology·F ProtasiP D Allen
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·Y Ogawa
Jul 8, 1992·Biochimica Et Biophysica Acta·A Zarka, V Shoshan-Barmatz
Feb 28, 1990·Biochemical and Biophysical Research Communications·J H CollinsN Ikemoto
Mar 13, 1987·Biochemical and Biophysical Research Communications·F A LaiG Meissner
Nov 11, 2017·The Journal of General Physiology·Gerhard Meissner
Jan 22, 1992·Proceedings. Biological Sciences·A F DulhuntyC Stanhope
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·N IkemotoL G Mészáros
May 24, 2019·The Journal of Physiology·Ji JingYubin Zhou
Sep 25, 1998·Cardiovascular Research·S T Rapundalo
Oct 1, 1994·Journal of Muscle Research and Cell Motility·H K MotoikeN R Brandt
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Stefano PerniKurt G Beam
Aug 13, 1999·Journal of Applied Physiology·T G Favero
Feb 1, 1992·Journal of Muscle Research and Cell Motility·A J Williams
Aug 1, 1988·Journal of Muscle Research and Cell Motility·R M KawamotoA H Caswell
Dec 1, 1990·Journal of Muscle Research and Cell Motility·E DamianiA Margreth
Oct 1, 1989·The American Journal of Physiology·J R MickelsonC F Louis
Mar 1, 1988·The American Journal of Physiology·G Salviati, P Volpe

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