PMID: 5271175Jun 1, 1970

Isolation, characterization, and location of a donor-acceptor unit from cross-linked fibrin

Proceedings of the National Academy of Sciences of the United States of America
R Chen, R F Doolittle

Abstract

The cross-linking systems of bovine and human fibrins were studied by the introduction of a radioactive substitute donor as an inhibitor of fibrin cross-linking, separation of the constituent polypeptide chains after sulfitolysis, and tryptic digestion of the labeled gamma-chains. The information gathered from this approach enabled us to isolate and characterize the complete donor-acceptor unit in tryptic digests of fibrin gamma-gamma cross-linked systems. In both bovine and human fibrin, this kind of cross-linking is accomplished by reciprocal bridging between overlapping carboxy-terminal segments of neighboring gamma-chains. The amino acid sequence of the carboxy-terminal heptadecapeptide of the bovine gamma-chain was determined and an alignment of the corresponding region of the human gamma-chain established.

References

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Citations

Dec 1, 1992·Protein Science : a Publication of the Protein Society·R F Doolittle
Mar 28, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·J P KamerlingS K Wadman
Mar 1, 1978·Thrombosis Research·G G LindseyL Purves
Apr 10, 1996·Biochimica Et Biophysica Acta·B M Fraij, R A Gonzales
Nov 17, 1988·Biochimica Et Biophysica Acta·R Procyk, B Blombäck
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J J PisanoY Nagai
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A Ichinose, E W Davie
Dec 8, 1972·Annals of the New York Academy of Sciences·R F DoolittleG L Wooding
Dec 8, 1972·Annals of the New York Academy of Sciences·S Iwanaga, M Okude
Jul 1, 1971·The Journal of Clinical Investigation·M L SchwartzP A McKee
Jul 21, 1972·Biochimica Et Biophysica Acta·T Takagi, K Konishi
Nov 19, 1971·Biochimica Et Biophysica Acta·M Okude, S Iwanaga
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Sep 8, 2004·Journal of Molecular and Cellular Cardiology·Francisco MarínVicente Vicente
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