PMID: 6964401Oct 1, 1982Paper

Isolation, characterization, and purification to homogeneity of a rat brain protein (GABA-modulin)

Proceedings of the National Academy of Sciences of the United States of America
A GuidottiE Costa

Abstract

gamma-Aminobutyric acid (GABA)-modulin is a brain neuropeptide that appears to modulate specific high-affinity (20 nM) GABA recognition sites in brain. When added to crude synaptic membranes this peptide inhibits binding of [3H]GABA to the high-affinity site and prevents facilitation of [3H]diazepam binding elicited by GABA. GABA-modulin has been purified to homogeneity by ammonium sulfate precipitation, gel chromatography, and reverse-phase HPLC. Homogeneity was confirmed by a variety of means, including chromatography under four different HPLC conditions, two different polyacrylamide gel electrophoreses, and end group analysis. Purified GABA-modulin contains approximately 126 amino acids and has a molecular weight of 16,500. The GABA-modulin molecule contains an abundance of hydrophilic basic residues, and neither cysteine nor GABA is present. End group analyses of GABA-modulin showed that histidine is the free COOH terminus and the NH2 terminus is blocked. GABA-modulin specifically blocked both [3H]GABA binding to synaptic membranes (IC50, 0.5 microM) and GABA-stimulated [3H]diazepam binding; the binding of [3H]GABA to low-affinity sites was not affected.

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Citations

Dec 1, 1983·Neurochemical Research·P GuarneriF Piccoli
Oct 1, 1985·Biochemical Pharmacology·E Costa, A Guidotti
May 6, 1983·European Journal of Pharmacology·A ConcasG Biggio
Sep 3, 1984·European Journal of Pharmacology·G Maksay, M K Ticku
Jan 1, 1983·Neurochemistry International·M Hamon, P Soubrié
Jan 1, 1995·Neurochemistry International·Y DaleziosT Valcana
Jul 1, 1994·Molecular and Cellular Endocrinology·J Fernández-AlvarezR Gomis
Dec 2, 1983·Neuroscience Letters·Y TatsuokaH Imura
Oct 21, 1994·Biochimica Et Biophysica Acta·H R KimM Y Chung
Aug 15, 1998·Trends in Pharmacological Sciences·C Szabó, V L Dawson
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A GuidottiE Costa
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B C WiseE Costa
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M G CordaE Costa
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P FerreroE Costa
Sep 1, 1988·Neurochemical Research·C O'Neill, D C Williams
Jul 1, 1983·Journal of Neurochemistry·M G Corda, A Guidotti
Apr 1, 1997·Experimental Neurology·R S SidhuS S Seshia
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M Hoyumpa
Oct 13, 1983·Nature·E J Nestler, P Greengard
Oct 19, 2010·Neuropharmacology·S J Enna

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