PMID: 40545Aug 1, 1979

Isolation, characterization and the role of rabbit testicular arysulphatase A in fertilization

The Biochemical Journal
A A Farooqui, P N Srivastava

Abstract

Arysulphatase A was purified from rabbit testis. The purification was accomplished by a four-step procedure involving (NH4)2SO4 fractionation, chromatography on DEAE-cellulose, SP(sulphopropyl)-Sephadex and affinity chromatography on concanavalin A-Sepharose. The specific activity of purified preparation was 135 mumol/min per mg of protein, which represented an increase of 900-fold above that of the crude homogenate. The purified enzyme (20-50 micrograms) was found to move electrophoretically as a single band on polyacrylamide gel at pH 7.2 and 8.4. The homogeneous enzyme was shown to be a glycoprotein with 0.8% (w/w) of N-acetylneuraminic acid and 20% neutral sugar. The treatment of purified enzyme with bacterial neuraminidase had no effect on enzyme activity or kinetic properties, but it changed the elution prolife of rabbit testis arylsulphatase A through DEAE-Sephadex. The purified enzyme was strongly inhibited by Cu2+, Fe3+ and Ag+. It hydrolysed several sulphate esters including cerebroside 3-sulphate, ascorbic acid 2-sulphate and steroid sulphates. Pure arysulphatase was effective in dispersing the cumulus cells of rabbit ova.

References

Jan 1, 1987·Andrologia·V Ninjoor, P N Srivastava
Jan 1, 1984·Neurochemical Pathology·A A Farooqui, L A Horrocks
Aug 1, 1994·Molecular Reproduction and Development·A RethinaswamyP N Srivastava
Feb 1, 1995·Molecular Reproduction and Development·Y Y YuanP N Srivastava
Oct 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·G LavyA H DeCherney
Dec 15, 1983·Experientia·A A FarooquiW L Hanson
Jan 15, 1982·Experientia·B W HarrisonK J Abbas

Related Concepts

Benign Neoplasm of Testis
Polyacrylamide Gels
Neoplasm of Uncertain or Unknown Behavior of Testis
Glycoproteins
Hepatitis A
Chromatography
Neuraminidase
Ascorbic Acid Measurement
Cerebrosides
Chromatography, Agarose

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