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Isolation, chemical, and physical properties of alpha-1-antitrypsin

Biochemistry

Feb 24, 1976

P Musiani, Thomas B Tomasi

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Abstract

A method of isolation of alpha-1-antitrypsin (alpha-1-AT) in good yield from normal human plasma is described. A key step was affinity chromatography employing an antiserum which had been depleted of alpha-1-AT antibodies. The final preparations were homogeneous by immunological and phy...read more

Mentioned in this Paper

Zemaira
Hydrogen-Ion Concentration
SDS-PAGE
Chromatography, Affinity
Spectrophotometry, Ultraviolet
Prolastin
Hexoses
Protein Denaturation
Paper Details
References
    • References18
    • Citations17
    • References18
    • Citations17
  • Isolation, chemical, and physical properties of alpha-1-antitrypsin

    Biochemistry

    Feb 24, 1976

    P Musiani, Thomas B Tomasi

    PMID: 2286

    DOI: 10.1021/bi00649a011

    Abstract

    A method of isolation of alpha-1-antitrypsin (alpha-1-AT) in good yield from normal human plasma is described. A key step was affinity chromatography employing an antiserum which had been depleted of alpha-1-AT antibodies. The final preparations were homogeneous by immunological and phy...read more

    Mentioned in this Paper

    Zemaira
    Hydrogen-Ion Concentration
    SDS-PAGE
    Chromatography, Affinity
    Spectrophotometry, Ultraviolet
    Paper Details
    References
    • References18
    • Citations17
    • References18
    • Citations17
  • Get paper from

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