PMID: 7524354Oct 1, 1994Paper

Isolation, culture, and characterization of rat lung microvascular endothelial cells

The American Journal of Physiology
J C MageeK S Guice

Abstract

Highly pure primary cultures of rat lung microvascular endothelial cells were obtained from peripheral lung tissue using a combination of selective culture strategies. The cells had a characteristic morphology consistent with an endothelial origin and were positive for a number of endothelial cell markers, including uptake of fluorescent acetylated lactate dehydrogenase, binding of the lectin Bandeiraea simplicifolia I, and positive immunofluorescence staining with two endothelial cell monoclonal antibodies. The cells behaved as microvascular endothelial cells using an in vitro angiogenesis assay. This isolation method provides a simple method for culturing the pulmonary microvasculature of the rat and these studies support the idea that endothelial cells from different vessels exhibit phenotypic heterogeneity. This method should prove useful for studying specialized endothelial cell function and differentiation in vitro.

Citations

May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eiichi SakuraiYorihisa Tanaka
Jul 5, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Cecile J FavreDonald M McDonald
May 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jacqueline E TestaJan E Schnitzer
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia R BachillerJesse D Roberts
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y ZhaoR Silbajoris
Mar 16, 2006·The Journal of Pharmacy and Pharmacology·Yoshinori OchiaiYorihisa Tanaka
Sep 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Theresa A JohnJ Usha Raj
Nov 9, 2019·Cells·Özgür UsluSandip M Kanse
Aug 20, 2010·Biological Trace Element Research·Eiichi SakuraiYasuyuki Yagi
Jun 24, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Reece P StevensJi Young Lee

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