Isolation, culture, and characterization of the two cell subpopulations forming the rat decidua: differential gene expression for activin, follistatin, and decidual prolactin-related protein

Endocrinology
Y Gu, G Gibori

Abstract

The decidual tissue of the pregnant rat is formed primarily by two markedly different decidual cell populations located in the mesometrial and antimesometrial sites of the uterus. The antimesometrial decidua functions as an endocrine organ, secreting PRL-like hormones and expressing activin and follistatin. In contrast, the mesometrial decidua has no apparent endocrine activity, but secretes abundant alpha 2-macroglobulin. To determine whether the profound difference in morphology and gene expression is inherent to each cell population or whether it results from the position of these cells in the uterus and their subjection to regionally restricted information, we isolated the giant antimesometrial decidual cells from the small mesometrial decidual cells by elutriation. We examined, using Northern and reverse transcription-polymerase chain reaction analysis, the expression of PRL-related protein (DPRP), follistatin, activin, and alpha 2-macroglobulin in the two cell populations just after cell separation and after primary culture. We also examined, using coculture, the possible cross-talk between the two cell populations. In culture, the cells maintained the morphological appearance seen in the decidua; the mesometrial cells re...Continue Reading

Citations

Jan 13, 2005·Reproductive Biology and Endocrinology : RB&E·Eduardo A CallegariGeula Gibori
Aug 7, 2010·General and Comparative Endocrinology·Felicity J Bradshaw, Don Bradshaw
May 23, 2001·Biology of Reproduction·H C Keith HoB P Schick
Jun 19, 2002·American Journal of Reproductive Immunology : AJRI·S CaluwaertsF A Van Assche
Dec 23, 1999·The Journal of Biological Chemistry·A Prigent-TessierG Gibori
Nov 4, 1998·Frontiers in Neuroendocrinology·D J Phillips, D M de Kretser

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