PMID: 7015341Mar 1, 1981Paper

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats

Proceedings of the National Academy of Sciences of the United States of America
A L KierszenbaumL L Tres

Abstract

A method for the isolation and culture of epididymal epithelial cells obtained from pubertal and old adult rats is described. This method permits the establishment of primary cultures of these cells in monolayers from aggregates isolated from whole epididymides and major epididymal anatomical segments (caput, corpus, and cauda) after trypsin and collagenase digestions. A large number of cultured epididymal cells retain a differentiated function as demonstrated by the immunocytochemical and radioimmunoassay finding of acidic epididymal glycoprotein, a spermatozoa-coating protein secreted by the principal cells of rat epididymis. The proliferative potential of cultured epididymal cells obtained from pubertal and old adult donors can be documented by [3H]thymidine labeling and mitotic indices without significant loss of gene expression for acidic epididymal glycoprotein. Results of this study demonstrate that epididymal epithelial cells, consisting of a predominant population of principal cells, can be isolated, cultured, and maintained for up to 3 months.

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Citations

Mar 29, 2000·Molecular Reproduction and Development·C KirchhoffI Kascheike
Apr 30, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Ali LamaraYahia Chebloune
Jun 15, 2005·Journal of Molecular Biology·Yasuo ShikamotoHiroshi Mizuno
Feb 10, 2012·Molecular Biology and Evolution·Kartik SunagarAgostinho Antunes
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Daisuke KobayashiIkumi Tamai
Jul 20, 2012·General and Comparative Endocrinology·Renee ZayaChristopher A Pearl
Mar 17, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·María E PeichotoMarcelo L Santoro
Mar 31, 2009·Regulatory Peptides·Simona TafuriAlfredo Vittoria
Sep 9, 2004·Molecular and Cellular Endocrinology·A BritanJ R Drevet
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Yasuo Yamazaki, Takashi Morita
Oct 21, 2014·PLoS Neglected Tropical Diseases·Camila M AdadeThaïs Souto-Padrón
Aug 4, 2009·Molecular and Cellular Endocrinology·Thomas E Willnow, Anders Nykjaer
Feb 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M V LoboR M del Río
Mar 26, 2014·Anatomia, histologia, embryologia·G LiguoriN Mirabella
Apr 5, 2001·American Journal of Physiology. Cell Physiology·G P LeungC M Tse
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Jul 1, 1995·Andrologia·F Martínez-GarcíaM Nistal
Nov 1, 1983·Journal of Andrology·G E OlsonM C Orgebin-Crist

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