Isolation, endocrine regulation and transcript distribution of a putative primary JH-responsive gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)

Insect Biochemistry and Molecular Biology
Jeremy C BearfieldClaus Tittiger

Abstract

We isolated a cDNA of unknown function from a juvenile hormone III (JH III)-treated male midgut cDNA library prepared from the pine engraver beetle, Ips pini, and examined its genomic structure. The gene, tentatively named "Ipi10G08", encoded a 410 amino acid translation product that shared 26-37% identity with unannotated matches from several insects. Semi-quantitative RT-PCR analysis of Ipi10G08 following application of a 10 microg dose of JH III demonstrated an early induction for both male and female beetles, with transcripts being detectable after 45 min. An expression profile of male midgut tissue indicated Ipi10G08 transcript levels reach a maximum induction of approximately 22.5-fold control levels at 4h post-treatment. Tissue distribution studies displayed a large induction of Ipi10G08 mRNA in the alimentary canal of JH III-treated beetles, especially in males. A dose curve from both sexes suggested there may be a difference in the ability to respond to lower levels of JH III and immunoblot analysis indicated that although JH III highly induces transcript levels in females, protein levels are not similarly induced, while protein levels are induced in males. Ipi10G08 is likely a primary JH response gene and may provide ...Continue Reading

References

May 1, 1990·Archives of Biochemistry and Biophysics·V J WroblewskiB D Hammock
Oct 1, 1987·Molecular and Cellular Endocrinology·R Feyereisen, D E Farnsworth
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·A WennborgA von Gabain
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·W M Barnes
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 16, 1999·Insect Biochemistry and Molecular Biology·J A TillmanG J Blomquist
Jul 6, 2000·Insect Biochemistry and Molecular Biology·L I GilbertR M Roe
Jul 6, 2000·Insect Biochemistry and Molecular Biology·K G Davey
Aug 6, 2000·Developmental Biology·E B DubrovskyE M Berger
Jan 11, 2000·Nucleic Acids Research·E WingenderS Urbach
Oct 9, 2001·Bioinformatics·G E Tusnády, I Simon
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J SeyboldG J Blomquist
May 9, 2002·Molecular and Cellular Endocrinology·S ZhouV K Walker
Oct 9, 2002·Genome Research·Lewis Y GeerStephen H Bryant
Jan 18, 2003·Insect Biochemistry and Molecular Biology·E B DubrovskyE M Berger
Jun 26, 2003·Nucleic Acids Research·Ramu ChennaJulie D Thompson
Sep 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Diana E Wheeler, H F Nijhout
Dec 19, 2003·Nucleic Acids Research·Alex BatemanSean R Eddy
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Sep 18, 2004·Insect Molecular Biology·A R BarchukZ L P Simões
Dec 29, 2004·Trends in Endocrinology and Metabolism : TEM·Edward B Dubrovsky
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anna B GilgGary J Blomquist
Dec 31, 2005·Nucleic Acids Research·Ivica LetunicPeer Bork
Jan 13, 2006·Molecular and Cellular Endocrinology·Thomas A GorrGerald A LeBlanc
Feb 2, 2006·Molecular and Cellular Endocrinology·Damu R KethidiSubba R Palli
Oct 19, 2006·Insect Biochemistry and Molecular Biology·Pamela SandstromClaus Tittiger
Oct 25, 2007·The Journal of Biological Chemistry·Yiping LiSubba R Palli

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