Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus.

Molecular & General Genetics : MGG
P A WiersmaM M Moloney

Abstract

An acetolactate synthase gene was isolated and characterized from Brassica napus. This B. napus acetolactate synthase gene encodes a deduced polypeptide sequence of 637 amino acids which is 85% homologous to the corresponding proposed gene product from Arabidopsis thaliana. Peptide domains recently associated with herbicide resistance/sensitivity are conserved between the two sequences. From Southern analysis we conclude that the gene isolated is one member of a multigene acetolactate synthase gene family comprising four or five members. A probe spanning the presumptive transit peptide sequence of this gene was shown by Southern analysis to hybridize to a unique sequence in the B. napus genome. This unique probe was used to analyse DNA from B. campestris and B. oleracea, the presumed progenitors of B. napus. On the basis of restriction fragment length polymorphism, we conclude that the B. napus gene isolated here originated in B. campestris. Total acetolactate synthase-homologous transcripts were analysed in a variety of B. napus tissues, and showed preferential accumulation in rapidly growing material. The genomic clone was mutated in vitro at codon 173 to replace a proline residue with serine. This was re-introduced into plan...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Nov 1, 1974·Journal of Bacteriology·E D Ryan, G B Kohlhaw
Nov 26, 1984·Nucleic Acids Research·M Bevan
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N YadavS C Falco
Oct 1, 1984·Plant Physiology·D L ShanerM A Stidham
Aug 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G L Creason, R S Chaleff
Sep 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B S LandryR W Michelmore
Mar 1, 1988·Plant Molecular Biology·J C Rogers

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Citations

Dec 1, 1992·Protein Science : a Publication of the Protein Society·P BorkE Sonnhammer
Dec 19, 2000·Biochemical and Biophysical Research Communications·C K Chong, J D Choi
Apr 1, 1992·Plant Molecular Biology·L Y FangM Lillis
Oct 17, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cecilia GhioCarlos A Sala
Jul 10, 1998·Biochimica Et Biophysica Acta·D ChipmanJ V Schloss
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B KeithG R Fink
Mar 11, 1990·Nucleic Acids Research
May 26, 2010·The Plant Journal : for Cell and Molecular Biology·Hao ChenLiming Xiong
Jan 24, 2015·Journal of Experimental Botany·Bryan McKersie
Sep 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C T HarmsR W Briggs
Mar 28, 2021·Pesticide Biochemistry and Physiology·Zhonghua SunMingshan Ji

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