Isolation, Expression, and Promoter Analysis of GbWRKY2: A Novel Transcription Factor Gene from Ginkgo biloba

International Journal of Genomics
Yong-Ling LiaoFeng Xu

Abstract

WRKY transcription factor is involved in multiple life activities including plant growth and development as well as biotic and abiotic responses. We identified 28 WRKY genes from transcriptome data of Ginkgo biloba according to conserved WRKY domains and zinc finger structure and selected three WRKY genes, which are GbWRKY2, GbWRKY16, and GbWRKY21, for expression pattern analysis. GbWRKY2 was preferentially expressed in flowers and strongly induced by methyl jasmonate. Here, we cloned the full-length cDNA and genomic DNA of GbWRKY2. The full-length cDNA of GbWRKY2 was 1,713 bp containing a 1,014 bp open reading frame encoding a polypeptide of 337 amino acids. The GbWRKY2 genomic DNA had one intron and two exons. The deduced GbWRKY2 contained one WRKY domain and one zinc finger motif. GbWRKY2 was classified into Group II WRKYs. Southern blot analysis revealed that GbWRKY2 was a single copy gene in G. biloba. Many cis-acting elements related to hormone and stress responses were identified in the 1,363 bp-length 5'-flanking sequence of GbWRKY2, including W-box, ABRE-motif, MYBCOREs, and PYRIMIDINE-boxes, revealing the molecular mechanism of upregulated expression of GbWRKY2 by hormone and stress treatments. Further functional char...Continue Reading

References

Jan 1, 1989·Molecular & General Genetics : MGG·A ShirsatD Boulter
Mar 13, 1999·The Plant Journal : for Cell and Molecular Biology·S Yanagisawa, R J Schmidt
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·A RoseU Wienand
Aug 10, 2000·Plant & Cell Physiology·L Lopez-Molina, N H Chua
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 16, 2002·Current Opinion in Plant Biology·Barbara N Kunkel, David M Brooks
Nov 13, 2002·Plant Physiology·Ludmila RizhskyRon Mittler
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Sean D SimpsonKazuko Yamaguchi-Shinozaki
Feb 27, 2003·Plant Molecular Biology·Jixin DongZhixiang Chen
Nov 18, 2005·Plant Physiology and Biochemistry : PPB·Guoan ShenKexuan Tang
Jun 10, 2006·The Plant Journal : for Cell and Molecular Biology·I Rubio-SomozaP Carbonero
Jul 20, 2006·Bioscience Reports·Zhongxiang DengKexuan Tang
Aug 22, 2006·Journal of Plant Physiology·Xiaoqiang LiuChengcai Chu
Apr 17, 2008·Plant & Cell Physiology·Rengasamy RamamoorthySrinivasan Ramachandran
May 1, 2009·Journal of Environmental Sciences (China)·Xingyuan HeYanan Ruan
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
Apr 26, 2011·Molecular Biology and Evolution·Yangyun ZouXun Gu
Jul 12, 2011·Current Opinion in Plant Biology·Regina AntoniPedro L Rodriguez
Oct 1, 2011·BMC Genomics·Jian LingBingyan Xie
May 13, 2014·Frontiers in Plant Science·Carles M LlorcaUlrike Zentgraf

❮ Previous
Next ❯

Citations

Jul 5, 2016·Frontiers in Plant Science·Ujjal J PhukanRakesh K Shukla

❮ Previous
Next ❯

Datasets Mentioned

BETA
L26924
KP987204
KP987205

Methods Mentioned

BETA
electrophoresis
Illumina Sequencing
RNA-Seq
PCR
X-ray
environmental stress
transgenic

Software Mentioned

BLASTP
CLUSTAL
NTI Suite
Sequence Detection System
TBLASTN
Genome Walker Universal Kit

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.