Isolation, follicular density, and culture of preantral follicles of buffalo fetuses of different ages

Animal Reproduction Science
S S D SantosO M Ohashi

Abstract

The aim of the present study was to determine the most desirable ovarian tissue section thickness to isolate preantral follicles (Experiment I), determine follicular density (follicles/mm(2) of cortex) of ovaries of fetal buffalo of different ages (Experiment II), and cultivate preantral follicles of buffalo fetuses (Experiment III). In Experiment I, ovary sections with different thicknesses (25, 50, 75, and 100 microm) had 415.0+/-285.2, 457.5+/-341.9, 585.0+/-309.3, and 685.0+/-278.8 isolated preantral follicles, respectively. In Experiment II, the follicular density of 46 buffalo fetuses with ages between 3 and 8 months was estimated to be between 0 and 7220, with means of 0.0, 2070.7+/-2190.3, 2570.8+/-1796.6, 2298.1+/-2286.5, 1277.5+/-1074.9, and 643.6+/-543.9 throughout the age range studied. The follicular density of 5-month-old fetuses was greatest, coinciding with the largest number of follicles isolated at this age. In Experiment III, preantral follicles isolated from the ovaries of buffalo fetuses aged from 5 to 9 months old were cultivated individually for 7 days in four different media: basic medium (Minimal Essential Medium (MEM), 10% SFB, kanamycin, pyruvate, glutamine, hypoxanthine) with additional ITS and FSH 0...Continue Reading

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Citations

Jan 29, 2008·Zygote : the Biology of Gametes and Early Embryos·P S P GuptaJ P Ravindra
Aug 21, 2010·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·G Taru SharmaG Sai Kumar
Apr 21, 2010·Reproduction in Domestic Animals = Zuchthygiene·G Taru SharmaG Sai Kumar
Apr 21, 2010·Reproduction in Domestic Animals = Zuchthygiene·S S D SantosO M Ohashi
Dec 6, 2008·Reproduction in Domestic Animals = Zuchthygiene·R G MondadoriS N Báo
Dec 14, 2004·Fertility and Sterility·Belen Martinez-MadridJacques Donnez
Sep 12, 2018·Reproduction in Domestic Animals = Zuchthygiene·Nathalie Jiatsa DonfackAna Paula Ribeiro Rodrigues
Aug 16, 2019·Zygote : the Biology of Gametes and Early Embryos·Lívia Batista CamposAlexandre Rodrigues Silva
Apr 27, 2017·Reproduction, Fertility, and Development·K A AlvesE L Gastal

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